PMID- 26725912
OWN - NLM
STAT- MEDLINE
DCOM- 20161017
LR  - 20181202
IS  - 2042-7158 (Electronic)
IS  - 0022-3573 (Linking)
VI  - 68
IP  - 1
DP  - 2016 Jan
TI  - Consequence of hyperhomocysteinaemia on alpha1-adrenoceptor-mediated contraction in 
      the rat corpus cavernosum: the role of reactive oxygen species.
PG  - 63-75
LID - 10.1111/jphp.12486 [doi]
AB  - OBJECTIVES: Our main objective was to investigate the mechanisms underlying the 
      effects of hyperhomocysteinaemia (HHcy) on contractile response mediated by 
      alpha1-adrenoceptors in the rat corpus cavernosum. METHODS: Concentration-response 
      curves for phenylephrine (PE) were obtained in strips of corpus cavernosum, in 
      absence or after incubation with tiron, tempol or polyethylene glycol 
      (PEG)-catalase combined or not with tempol. We also measured the superoxide anion 
      (O2(-)) and hydrogen peroxide (H2O2) generation, superoxide dismutase (SOD) and 
      catalase activity and alpha-actin expression in rat corpus cavernosum from both 
      groups. KEY FINDINGS: HHcy increased PE-induced contraction in cavernosal strips. 
      Tiron, PEG-catalase or tempol increased PE-induced contraction in strips from 
      control rats, but it was not altered by tiron or PEG-catalase in HHcy rats, 
      whereas tempol reduced this response. The combination of PEG-catalase and tempol 
      did not alter the contractile response to PE in both groups. HHcy increased O2(-) 
      generation and SOD activity, whereas H2O2 concentration was reduced. Finally, 
      HHcy did not alter catalase activity or expression of alpha-actin. CONCLUSIONS: The 
      major new finding from this study is that HHcy induced a marked increase in 
      PE-induced contraction in rat corpus cavernosum by a mechanism that involves 
      increased O2(-) generation and it could play a role in the pathogenesis of 
      erectile dysfunction associated with HHcy.
CI  - (c) 2015 Royal Pharmaceutical Society.
FAU - Coco, Hariane
AU  - Coco H
AD  - Departament of Pharmacology, Faculdade de Medicina de Ribeirao Preto, Ribeirao 
      Preto, SP, Brazil.
FAU - Pernomian, Larissa
AU  - Pernomian L
AD  - Laboratory of Vascular Injury, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Ribeirao Preto, SP, Brazil.
FAU - Marchi, Katia C
AU  - Marchi KC
AD  - Departament of Pharmacology, Faculdade de Medicina de Ribeirao Preto, Ribeirao 
      Preto, SP, Brazil.
FAU - Gomes, Mayara S
AU  - Gomes MS
AD  - Laboratory of Vascular Injury, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Ribeirao Preto, SP, Brazil.
FAU - de Andrade, Claudia R
AU  - de Andrade CR
AD  - Department of Pathology and Legal Medicine, Faculdade de Medicina de Ribeirao 
      Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil.
FAU - Ramalho, Leandra N Z
AU  - Ramalho LN
AD  - Nucleus of Applied Bioprospection and Molecular Research, Faculdades Inta, 
      Fortaleza, CE, Brazil.
FAU - Tirapelli, Carlos R
AU  - Tirapelli CR
AD  - Laboratory of Pharmacology, Escola de Enfermagem de Ribeirao Preto, Ribeirao 
      Preto, SP, Brazil.
FAU - de Oliveira, Ana M
AU  - de Oliveira AM
AD  - Laboratory of Vascular Injury, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Ribeirao Preto, SP, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160104
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Actins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Adrenergic, alpha-1)
RN  - 0 (catalase-polyethylene glycol)
RN  - 11062-77-4 (Superoxides)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Catalase/metabolism
MH  - Erectile Dysfunction/metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - Hyperhomocysteinemia/*metabolism
MH  - Male
MH  - Muscle Contraction/drug effects/physiology
MH  - Muscle, Smooth/drug effects/metabolism
MH  - Penis/drug effects/*metabolism
MH  - Phenylephrine/pharmacology
MH  - Polyethylene Glycols/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/*metabolism
MH  - Receptors, Adrenergic, alpha-1/*metabolism
MH  - Superoxides/metabolism
OTO - NOTNLM
OT  - corpus cavernosum
OT  - hyperhomocysteinaemia
OT  - alpha1-adrenergic agonist and reactive oxygen species
EDAT- 2016/01/05 06:00
MHDA- 2016/10/19 06:00
CRDT- 2016/01/05 06:00
PHST- 2015/06/11 00:00 [received]
PHST- 2015/09/12 00:00 [accepted]
PHST- 2016/01/05 06:00 [entrez]
PHST- 2016/01/05 06:00 [pubmed]
PHST- 2016/10/19 06:00 [medline]
AID - 10.1111/jphp.12486 [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 2016 Jan;68(1):63-75. doi: 10.1111/jphp.12486. Epub 2016 Jan 
      4.