PMID- 26726028
OWN - NLM
STAT- MEDLINE
DCOM- 20170426
LR  - 20181202
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 17
IP  - 3
DP  - 2016 Sep 30
TI  - Immunologic properties of differentiated and undifferentiated mesenchymal stem 
      cells derived from umbilical cord blood.
PG  - 289-97
LID - 10.4142/jvs.2016.17.3.289 [doi]
AB  - The expression of immunogenic markers after differentiation of umbilical cord 
      blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and 
      requires extensive in vitro and in vivo testing for clinical application. The 
      expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested 
      by Fluorescence-activated cell sorting analysis and immunocytochemical staining. 
      The undifferentiated MSC were moderately positive for HLA-ABC, but almost 
      completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed 
      neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly 
      affected by the allogeneic lymphocytes stimulated with concanavalin A. The 
      responder lymphocytes showed no significant decrease in proliferation in the 
      presence of the MSC, but the apoptosis rate of the lymphocytes was increased in 
      the presence of MSC. Taken together, these findings indicate that UCB-derived MSC 
      differentiated to chondrocytes expressed less HLA class I and no class II 
      antigens. The MSC showed an immunomodulatory effect on the proliferation and 
      apoptosis of allogeneic lymphocytes. These data suggest that the differentiated 
      and undifferentiated allogeneic MSC derived from umbilical cord blood can be a 
      useful candidate for allogeneic cell therapy and transplantation without a major 
      risk of rejection.
FAU - Lee, Hyo-Jong
AU  - Lee HJ
AD  - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, 
      Korea.
AD  - Human Biotech Co. Ltd., Jinju 52839, Korea.
FAU - Kang, Kyung-Sun
AU  - Kang KS
AD  - Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National 
      University, Seoul 08826, Korea.
FAU - Kang, Sun-Young
AU  - Kang SY
AD  - Gyeongnam Wildlife Center, Gyeongsang National University, Jinju 52828, Korea.
FAU - Kim, Hyung-Sik
AU  - Kim HS
AD  - Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National 
      University, Seoul 08826, Korea.
FAU - Park, Se-Jin
AU  - Park SJ
AD  - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, 
      Korea.
FAU - Lee, Seung-Yong
AU  - Lee SY
AD  - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, 
      Korea.
FAU - Kim, Kwang-Dong
AU  - Kim KD
AD  - Division of Applied Life Science, Gyeongsang National University, Jinju 52828, 
      Korea.
FAU - Lee, Hee-Chun
AU  - Lee HC
AD  - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, 
      Korea.
FAU - Park, Ji-Kwon
AU  - Park JK
AD  - School of Medicine, Gyeongsang National University, Jinju 52828, Korea.
FAU - Paik, Won-Young
AU  - Paik WY
AD  - School of Medicine, Gyeongsang National University, Jinju 52828, Korea.
FAU - Lee, Lyon
AU  - Lee L
AD  - College of Veterinary Medicine, Western University, Pomona, CA 91766-1854, USA.
FAU - Yeon, Seong-Chan
AU  - Yeon SC
AD  - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, 
      Korea.
AD  - Gyeongnam Wildlife Center, Gyeongsang National University, Jinju 52828, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Fetal Blood/*cytology
MH  - Flow Cytometry
MH  - HLA Antigens/genetics/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation/*veterinary
MH  - Mesenchymal Stem Cells/cytology/*immunology
MH  - Mice
PMC - PMC5037295
OTO - NOTNLM
OT  - apoptosis
OT  - human leukocyte antigen
OT  - human umbilical cord blood
OT  - immunophenotypes
OT  - mesenchymal stem cells
COIS- There is no conflict of interest.
EDAT- 2016/01/05 06:00
MHDA- 2017/04/27 06:00
PMCR- 2016/09/01
CRDT- 2016/01/05 06:00
PHST- 2015/09/07 00:00 [received]
PHST- 2015/11/21 00:00 [revised]
PHST- 2015/12/30 00:00 [accepted]
PHST- 2016/01/05 06:00 [entrez]
PHST- 2016/01/05 06:00 [pubmed]
PHST- 2017/04/27 06:00 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - jvs.2015.091 [pii]
AID - 10.4142/jvs.2016.17.3.289 [doi]
PST - ppublish
SO  - J Vet Sci. 2016 Sep 30;17(3):289-97. doi: 10.4142/jvs.2016.17.3.289.