PMID- 26726951
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20191126
IS  - 1944-9917 (Electronic)
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 30
IP  - 2
DP  - 2016 Feb
TI  - Divergent Regulation of ER and Kiss Genes by 17beta-Estradiol in Hypothalamic ARC 
      Versus AVPV Models.
PG  - 217-33
LID - 10.1210/me.2015-1189 [doi]
AB  - Kisspeptin (Kiss) and G-protein-coupled receptor (Gpr)54 have emerged as key 
      regulators of reproduction. 17beta-estradiol (E2)-mediated regulation of these 
      neurons is nuclei specific, where anteroventral periventricular (AVPV) Kiss 
      neurons are positively regulated by E2, whereas arcuate nucleus (ARC) neurons are 
      inhibited. We have generated immortalized Kiss cell lines from male and female 
      adult-derived murine hypothalamic primary culture, as well as cell lines from 
      microdissected AVPV and ARC from female Kiss-green fluorescent protein (GFP) 
      mice. All exhibit endogenous Kiss-1 expression, estrogen receptors (ER)s (ERalpha, 
      ERbeta, and Gpr30), as well as known markers of AVPV Kiss neurons in the mHypoA-50 
      and mHypoA-Kiss/GFP-4, vs markers of ARC Kiss neurons in the mHypoA-55 and the 
      mHypoA-Kiss/GFP-3 lines. There was an increase in Kiss-1 mRNA expression at 24 
      hours in the AVPV lines and a repression of Kiss-1 mRNA at 4 hours in the ARC 
      lines. An E2-mediated decrease in ERalpha mRNA expression at 24 hours in the AVPV 
      cell lines was detected, and a significant decrease in Gpr30, ERalpha, and ERbeta mRNA 
      levels at 4 hours in the ARC cell lines was evident. ER agonists and antagonists 
      determined the specific ERs responsible for mediating changes in gene expression. 
      In the AVPV, ERalpha is required but not ERbeta or GPR30, vs the ARC Kiss-expressing 
      cell lines that require GPR30, and either ERalpha and/or ERbeta. We determined cAMP 
      response element-binding protein 1 was necessary for the down-regulation of 
      Kiss-1 mRNA expression using small interfering RNA knockdown in the ARC cell 
      model. These studies elucidate some of the molecular events involved in the 
      differential E2-mediated regulation of unique and specific Kiss neuronal models.
FAU - Treen, Alice K
AU  - Treen AK
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Luo, Vicky
AU  - Luo V
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Chalmers, Jennifer A
AU  - Chalmers JA
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Dalvi, Prasad S
AU  - Dalvi PS
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Tran, Dean
AU  - Tran D
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Ye, Wenqing
AU  - Ye W
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Kim, Ginah L
AU  - Kim GL
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Friedman, Zoey
AU  - Friedman Z
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
FAU - Belsham, Denise D
AU  - Belsham DD
AD  - Departments of Physiology (A.K.T., V.L., J.A.C., P.S.D., D.T., W.Y., G.L.K., 
      Z.F., D.D.B.), Medicine (D.D.B.), and Obstetrics and Gynaecology (D.D.B.), 
      University of Toronto, and Division of Cellular and Molecular Biology (D.D.B.), 
      Toronto General Hospital Research Institute, University Health Network, Toronto, 
      Ontario, Canada M5S 1A8.
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160104
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (GPER1 protein, mouse)
RN  - 0 (Kisspeptins)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Transcription Factor AP-1)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/drug effects/*metabolism
MH  - Binding Sites
MH  - Cell Line
MH  - Cell Membrane/drug effects/metabolism
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Gene Knockdown Techniques
MH  - Green Fluorescent Proteins/metabolism
MH  - Hypothalamus, Anterior/drug effects/*metabolism
MH  - Kisspeptins/*genetics/metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Models, Biological
MH  - Neurons/drug effects/metabolism
MH  - Peptides/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Estrogen/genetics/*metabolism
MH  - Receptors, G-Protein-Coupled/genetics/metabolism
MH  - Sex Characteristics
MH  - Transcription Factor AP-1/metabolism
PMC - PMC5414652
EDAT- 2016/01/05 06:00
MHDA- 2016/11/01 06:00
PMCR- 2017/02/01
CRDT- 2016/01/05 06:00
PHST- 2016/01/05 06:00 [entrez]
PHST- 2016/01/05 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - ME-15-1189 [pii]
AID - 10.1210/me.2015-1189 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2016 Feb;30(2):217-33. doi: 10.1210/me.2015-1189. Epub 2016 Jan 
      4.