PMID- 26727933
OWN - NLM
STAT- MEDLINE
DCOM- 20161111
LR  - 20181113
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 33
IP  - 2
DP  - 2016 Feb
TI  - Pronuclear removal of tripronuclear zygotes can establish heteroparental normal 
      karyotypic human embryonic stem cells.
PG  - 255-63
LID - 10.1007/s10815-015-0634-8 [doi]
AB  - PURPOSE: This study aimed to derive heteroparental normal karyotypic human 
      embryonic stem cells (hESCs) from microsurgically corrected tripronuclear (3PN) 
      zygotes. METHODS: After sequential culture for 5-6 days, embryos developed from 
      microsurgically corrected 3PN zygotes were analyzed by fluorescence in situ 
      hybridization (FISH) using probes for chromosomes 17, X and Y. Intact 3PN zygotes 
      from clinical in vitro fertilization (IVF) cycles were cultured as the control 
      group. The inner cell mass (ICM) of blastocysts that developed from 
      microsurgically corrected 3PN zygotes was used to derive hESC lines, and the stem 
      cell characteristics of these lines were evaluated. G-banding analysis was 
      adopted to identify the karyotype of the hESC line, and the heteroparental 
      inheritance of the hESC line was analyzed by DNA fingerprinting analysis. 
      RESULTS: The blastocyst formation rate (13.5 %) of the microsurgically corrected 
      3PN zygotes was significantly higher (P < 0.05) than that of intact 3PN zygotes 
      (8.7 %). The diploid rate of the blastocysts (55.0 %) was significantly higher 
      (P < 0.05) than that of the arrested cleavage-stage embryos (18.4 %) in 
      microsurgically corrected 3PN zygotes. The triploid rate of the microsurgically 
      corrected 3PN zygotes (5.7 %) was significantly lower (P < 0.01) than that of 
      intact 3PN zygotes (19.4 %). Furthermore, we established one heteroparental 
      normal karyotypic hESC line from the microsurgically corrected tripronuclear 
      zygotes. CONCLUSIONS: Pronuclear removal can effectively remove the surplus 
      chromosome set of 3PN zygotes. A combination of pronuclear removal and blastocyst 
      culture enables the selection of diploidized blastocysts from which 
      heteroparental normal karyotypic hESC lines can be derived.
FAU - Liao, Hong-Qing
AU  - Liao HQ
AD  - The Second Affiliated Hospital of South China University, 30th Jie-Fang Road, 
      Hengyang, Hunan, 421001, China. potlhq@gmail.com.
FAU - OuYang, Qi
AU  - OuYang Q
AD  - Institute of Reproductive and Stem Cell Engineering, Central South University, 
      Changsha, Hunan, 410078, China.
AD  - National Engineering and Research Center of Human Stem Cell, Changsha, Hunan, 
      410078, China.
FAU - Zhang, Shuo-Ping
AU  - Zhang SP
AD  - Institute of Reproductive and Stem Cell Engineering, Central South University, 
      Changsha, Hunan, 410078, China.
AD  - CITIC-Xiangya Reproductive and Genetic Hospital, Changsha, Hunan, 410078, China.
FAU - Cheng, De-Hua
AU  - Cheng DH
AD  - CITIC-Xiangya Reproductive and Genetic Hospital, Changsha, Hunan, 410078, China.
FAU - Lu, Guang-Xiu
AU  - Lu GX
AD  - Institute of Reproductive and Stem Cell Engineering, Central South University, 
      Changsha, Hunan, 410078, China.
AD  - National Engineering and Research Center of Human Stem Cell, Changsha, Hunan, 
      410078, China.
AD  - CITIC-Xiangya Reproductive and Genetic Hospital, Changsha, Hunan, 410078, China.
FAU - Lin, Ge
AU  - Lin G
AD  - Institute of Reproductive and Stem Cell Engineering, Central South University, 
      Changsha, Hunan, 410078, China. linge_csu@yahoo.com.
AD  - National Engineering and Research Center of Human Stem Cell, Changsha, Hunan, 
      410078, China. linge_csu@yahoo.com.
AD  - CITIC-Xiangya Reproductive and Genetic Hospital, Changsha, Hunan, 410078, China. 
      linge_csu@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160104
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (G-Bond)
RN  - 0 (Methacrylates)
SB  - IM
MH  - Blastocyst/*cytology
MH  - Cell Nucleus/*genetics
MH  - DNA Fingerprinting
MH  - Human Embryonic Stem Cells/*cytology/transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Methacrylates
MH  - Microtechnology
MH  - Zygote/*cytology/growth & development
PMC - PMC4759007
OTO - NOTNLM
OT  - Blastocyst culture
OT  - Heteroparental inheritance
OT  - Pronuclear removal
OT  - Tripronuclear zygotes
OT  - hESCs
EDAT- 2016/01/06 06:00
MHDA- 2016/11/12 06:00
PMCR- 2017/02/01
CRDT- 2016/01/06 06:00
PHST- 2015/07/31 00:00 [received]
PHST- 2015/12/07 00:00 [accepted]
PHST- 2016/01/06 06:00 [entrez]
PHST- 2016/01/06 06:00 [pubmed]
PHST- 2016/11/12 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - 10.1007/s10815-015-0634-8 [pii]
AID - 634 [pii]
AID - 10.1007/s10815-015-0634-8 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2016 Feb;33(2):255-63. doi: 10.1007/s10815-015-0634-8. 
      Epub 2016 Jan 4.