PMID- 26729343
OWN - NLM
STAT- MEDLINE
DCOM- 20161103
LR  - 20220331
IS  - 1613-2246 (Electronic)
IS  - 0021-5155 (Linking)
VI  - 60
IP  - 2
DP  - 2016 Mar
TI  - Changes in morphology and visual function over time in mouse models of retinal 
      degeneration: an SD-OCT, histology, and electroretinography study.
PG  - 111-25
LID - 10.1007/s10384-015-0422-0 [doi]
AB  - PURPOSE: To examine the long-term natural course of retinal degeneration in rd10 
      and rd12 mice using serial spectral-domain optical coherence tomography (SD-OCT), 
      electroretinography/electroretinograms (ERGs), and histological analysis. 
      METHODS: Photoreceptor layer thickness and the ability to visualize photoreceptor 
      ellipsoid zones were analyzed using SD-OCT images, and these images were compared 
      with hematoxylin and eosin-stained sections and electron microscopy images. The 
      a- and b-wave amplitudes of the ERGs were analyzed. RESULTS: In rd10 mice, the 
      photoreceptor layer thickness rapidly decreased, and the photoreceptor ellipsoid 
      zone was visible on SD-OCT images in 89 and 43 % of eyes of 21 and 33-day-old 
      mice, respectively. In rd12 mice, the photoreceptor layer gradually thinned, and 
      the ellipsoid zone remained visible in 92 % of eyes at 19 months. Electron 
      microscopy revealed that photoreceptor degeneration had occurred on the inner 
      side of the outer nuclear layer in 21-day-old rd10 and 7-month-old rd12 mice, 
      possibly due to autophagy mechanisms. Scotopic ERGs of rd10 mice showed a 
      diminished response at 21 days; at 33 days, no response was detectable. In rd12 
      mice, scotopic ERGs were undetectable at 28 days (stimulus intensity 3.0 
      cds/m(2)). Photopic ERGs were nearly undetectable in 28-day-old rd10 mice, but a 
      small b-wave was still recordable in 13-month-old rd12 mice. CONCLUSIONS: Our 
      results demonstrate that visual function deteriorated with photoreceptor 
      degeneration within 1 month in rd10 mice. In rd12 mice, however, the process of 
      visual function deterioration and photoreceptor degeneration was still in 
      progress at 13 months of age.
FAU - Hasegawa, Tomoko
AU  - Hasegawa T
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, 54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
FAU - Ikeda, Hanako O
AU  - Ikeda HO
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, 54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan. 
      hanakoi@kuhp.kyoto-u.ac.jp.
AD  - Institute for Advancement of Clinical and Translational Science, Kyoto University 
      Hospital, Kyoto, Japan. hanakoi@kuhp.kyoto-u.ac.jp.
FAU - Nakano, Noriko
AU  - Nakano N
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, 54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
FAU - Muraoka, Yuki
AU  - Muraoka Y
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, 54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
FAU - Tsuruyama, Tatsuaki
AU  - Tsuruyama T
AD  - Center for Anatomical Studies, Kyoto University Graduate School of Medicine, 
      Kyoto, Japan.
FAU - Okamoto-Furuta, Keiko
AU  - Okamoto-Furuta K
AD  - Center for Anatomical Studies, Kyoto University Graduate School of Medicine, 
      Kyoto, Japan.
FAU - Kohda, Haruyasu
AU  - Kohda H
AD  - Center for Anatomical Studies, Kyoto University Graduate School of Medicine, 
      Kyoto, Japan.
FAU - Yoshimura, Nagahisa
AU  - Yoshimura N
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, 54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160105
PL  - Japan
TA  - Jpn J Ophthalmol
JT  - Japanese journal of ophthalmology
JID - 0044652
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Electroretinography
MH  - Follow-Up Studies
MH  - Mice
MH  - Retina/*pathology
MH  - Retinal Degeneration/*physiopathology
MH  - Tomography, Optical Coherence
MH  - Visual Acuity/*physiology
OTO - NOTNLM
OT  - Longitudinal study
OT  - Retinal degeneration
OT  - Spectral-domain optical coherence tomography
OT  - rd10
OT  - rd12
EDAT- 2016/01/06 06:00
MHDA- 2016/11/04 06:00
CRDT- 2016/01/06 06:00
PHST- 2015/06/04 00:00 [received]
PHST- 2015/11/12 00:00 [accepted]
PHST- 2016/01/06 06:00 [entrez]
PHST- 2016/01/06 06:00 [pubmed]
PHST- 2016/11/04 06:00 [medline]
AID - 10.1007/s10384-015-0422-0 [pii]
AID - 10.1007/s10384-015-0422-0 [doi]
PST - ppublish
SO  - Jpn J Ophthalmol. 2016 Mar;60(2):111-25. doi: 10.1007/s10384-015-0422-0. Epub 
      2016 Jan 5.