PMID- 2673030
OWN - NLM
STAT- MEDLINE
DCOM- 19890928
LR  - 20190828
IS  - 0003-6072 (Print)
IS  - 0003-6072 (Linking)
VI  - 56
IP  - 1
DP  - 1989 May
TI  - Cloning of the genes encoding the two different glucose dehydrogenases from 
      Acinetobacter calcoaceticus.
PG  - 73-9
AB  - Glucose dehydrogenase (GDH) is a PQQ dependent bacterial enzyme which converts 
      aldoses to their corresponding acids. A. calcoaceticus contains two different PQQ 
      dependent glucose dehydrogenases designated GDH-A which is active in vivo and 
      GDH-B of which only in vitro activity can be shown. We cloned the genes coding 
      for the two GDH enzymes. The DNA sequences of both gdh genes were determined. 
      There is no obvious homology between gdhA and gdhB. Both GDH enzymes oxidize 
      D-glucose in vitro but disaccharides are specific GDH-B substrates and 
      2-deoxyglucose is specifically oxidized by GDH-A.
FAU - Cleton-Jansen, A M
AU  - Cleton-Jansen AM
AD  - Laboratory of Molecular Genetics, University of Leiden, The Netherlands.
FAU - Goosen, N
AU  - Goosen N
FAU - Vink, K
AU  - Vink K
FAU - van de Putte, P
AU  - van de Putte P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Antonie Van Leeuwenhoek
JT  - Antonie van Leeuwenhoek
JID - 0372625
RN  - 0 (DNA, Bacterial)
RN  - EC 1.1.- (Carbohydrate Dehydrogenases)
RN  - EC 1.1.1.- (Glucose Dehydrogenases)
RN  - EC 1.1.5.2 (glucose dehydrogenase (pyrroloquinoline-quinone))
SB  - IM
MH  - Acinetobacter/*enzymology/genetics
MH  - Carbohydrate Dehydrogenases/*genetics
MH  - Carbohydrate Metabolism
MH  - *Cloning, Molecular
MH  - DNA, Bacterial/genetics
MH  - Escherichia coli/genetics
MH  - *Genes, Bacterial
MH  - Glucose Dehydrogenases/*genetics/metabolism
MH  - Mutation
MH  - Plasmids
MH  - Pseudomonas/genetics
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 10.1007/BF00822586 [doi]
PST - ppublish
SO  - Antonie Van Leeuwenhoek. 1989 May;56(1):73-9. doi: 10.1007/BF00822586.