PMID- 26733976
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160106
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 6
DP  - 2015
TI  - Rapid Detection of K1 Hypervirulent Klebsiella pneumoniae by MALDI-TOF MS.
PG  - 1435
LID - 10.3389/fmicb.2015.01435 [doi]
LID - 1435
AB  - Hypervirulent strains of Klebsiella pneumoniae (hvKP) are genetic variants of K. 
      pneumoniae which can cause life-threatening community-acquired infection in 
      healthy individuals. Currently, methods for efficient differentiation between 
      classic K. pneumoniae (cKP) and hvKP strains are not available, often causing 
      delay in diagnosis and treatment of hvKP infections. To address this issue, we 
      devised a Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) 
      mass spectrometry (MS) approach for rapid identification of K1 hvKP strains. Four 
      standard algorithms, genetic algorithm (GA), support vector machine (SVM), 
      supervised neural network (SNN), and quick classifier (QC), were tested for their 
      power to differentiate between K1 and non-K1 strains, among which SVM was the 
      most reliable algorithm. Analysis of the receiver operating characteristic curves 
      of the interest peaks generated by the SVM model was found to confer highly 
      accurate detection sensitivity and specificity, consistently producing 
      distinguishable profiles for K1 hvKP and non-K1 strains. Of the 43 K. pneumoniae 
      modeling strains tested by this approach, all were correctly identified as K1 
      hvKP and non-K1 capsule type. Of the 20 non-K1 and 17 K1 hvKP validation 
      isolates, the accuracy of K1 hvKP and non-K1 identification was 94.1 and 90.0%, 
      respectively, according to the SVM model. In summary, the MALDI-TOF MS approach 
      can be applied alongside the conventional genotyping techniques to provide rapid 
      and accurate diagnosis, and hence prompt treatment of infections caused by hvKP.
FAU - Huang, Yonglu
AU  - Huang Y
AD  - Department of Clinical Microbiology, Second Affiliated Hospital of Zhejiang 
      University Hangzhou, China.
FAU - Li, Jiaping
AU  - Li J
AD  - Department of Clinical Microbiology, Second Affiliated Hospital of Zhejiang 
      University Hangzhou, China.
FAU - Gu, Danxia
AU  - Gu D
AD  - Department of Clinical Microbiology, Second Affiliated Hospital of Zhejiang 
      University Hangzhou, China.
FAU - Fang, Ying
AU  - Fang Y
AD  - Department of Clinical Microbiology, Second Affiliated Hospital of Zhejiang 
      University Hangzhou, China.
FAU - Chan, Edward W
AU  - Chan EW
AD  - Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology 
      Research Center, Hong Kong PolyU Shenzhen Research InstituteShenzhen, China; 
      State Key Lab of Chirosciences, Department of Applied Biology and Chemical 
      Technology, The Hong Kong Polytechnic UniversityHong Kong, China.
FAU - Chen, Sheng
AU  - Chen S
AD  - Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology 
      Research Center, Hong Kong PolyU Shenzhen Research InstituteShenzhen, China; 
      State Key Lab of Chirosciences, Department of Applied Biology and Chemical 
      Technology, The Hong Kong Polytechnic UniversityHong Kong, China.
FAU - Zhang, Rong
AU  - Zhang R
AD  - Department of Clinical Microbiology, Second Affiliated Hospital of Zhejiang 
      University Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20151221
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4685062
OTO - NOTNLM
OT  - K1 hvKP
OT  - MALDI-TOF MS
OT  - SVM model
OT  - rapid detection
OT  - typical spectra
EDAT- 2016/01/07 06:00
MHDA- 2016/01/07 06:01
PMCR- 2015/12/21
CRDT- 2016/01/07 06:00
PHST- 2015/08/26 00:00 [received]
PHST- 2015/12/01 00:00 [accepted]
PHST- 2016/01/07 06:00 [entrez]
PHST- 2016/01/07 06:00 [pubmed]
PHST- 2016/01/07 06:01 [medline]
PHST- 2015/12/21 00:00 [pmc-release]
AID - 10.3389/fmicb.2015.01435 [doi]
PST - epublish
SO  - Front Microbiol. 2015 Dec 21;6:1435. doi: 10.3389/fmicb.2015.01435. eCollection 
      2015.