PMID- 26734017
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160106
LR  - 20240324
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 6
DP  - 2015
TI  - The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity.
PG  - 1091
LID - 10.3389/fpls.2015.01091 [doi]
LID - 1091
AB  - Members of the ubiquitous COX11 (cytochrome c oxidase 11) protein family are 
      involved in copper delivery to the COX complex. In this work, we characterize the 
      Arabidopsis thaliana COX11 homolog (encoded by locus At1g02410). Western blot 
      analyses and confocal microscopy identified Arabidopsis COX11 as an integral 
      mitochondrial protein. Despite sharing high sequence and structural similarities, 
      the Arabidopsis COX11 is not able to functionally replace the Saccharomyces 
      cerevisiae COX11 homolog. Nevertheless, further analysis confirmed the hypothesis 
      that Arabidopsis COX11 is essential for COX activity. Disturbance of COX11 
      expression through knockdown (KD) or overexpression (OE) affected COX activity. 
      In KD lines, the activity was reduced by ~50%, resulting in root growth 
      inhibition, smaller rosettes and leaf curling. In OE lines, the reduction was 
      less pronounced (~80% of the wild type), still resulting in root growth 
      inhibition. Additionally, pollen germination was impaired in COX11 KD and OE 
      plants. This effect on pollen germination can only partially be attributed to COX 
      deficiency and may indicate a possible auxiliary role of COX11 in ROS metabolism. 
      In agreement with its role in energy production, the COX11 promoter is highly 
      active in cells and tissues with high-energy demand for example shoot and root 
      meristems, or vascular tissues of source and sink organs. In COX11 KD lines, the 
      expression of the plasma-membrane copper transporter COPT2 and of several copper 
      chaperones was altered, indicative of a retrograde signaling pathway pertinent to 
      copper homeostasis. Based on our data, we postulate that COX11 is a mitochondrial 
      chaperone, which plays an important role for plant growth and pollen germination 
      as an essential COX complex assembly factor.
FAU - Radin, Ivan
AU  - Radin I
AD  - Institute for Genetics, Department of Biology, Technische Universitat Dresden 
      Dresden, Germany.
FAU - Mansilla, Natanael
AU  - Mansilla N
AD  - Instituto de Agrobiotecnologia del Litoral-Consejo Nacional de Investigaciones 
      Cientificas y Tecnicas, Universidad Nacional del Litoral Santa Fe, Argentina.
FAU - Rodel, Gerhard
AU  - Rodel G
AD  - Institute for Genetics, Department of Biology, Technische Universitat Dresden 
      Dresden, Germany.
FAU - Steinebrunner, Iris
AU  - Steinebrunner I
AD  - Department of Biology, Technische Universitat Dresden Dresden, Germany.
LA  - eng
PT  - Journal Article
DEP - 20151218
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC4683207
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - COX complex
OT  - COX11 (cytochrome c oxidase 11)
OT  - copper chaperone
OT  - mitochondria
OT  - pollen development
OT  - root development
EDAT- 2016/01/07 06:00
MHDA- 2016/01/07 06:01
PMCR- 2015/01/01
CRDT- 2016/01/07 06:00
PHST- 2015/07/01 00:00 [received]
PHST- 2015/11/20 00:00 [accepted]
PHST- 2016/01/07 06:00 [entrez]
PHST- 2016/01/07 06:00 [pubmed]
PHST- 2016/01/07 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2015.01091 [doi]
PST - epublish
SO  - Front Plant Sci. 2015 Dec 18;6:1091. doi: 10.3389/fpls.2015.01091. eCollection 
      2015.