PMID- 26740789
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240324
IS  - 1310-2818 (Print)
IS  - 1314-3530 (Electronic)
IS  - 1310-2818 (Linking)
VI  - 29
IP  - 1
DP  - 2015 Jan 2
TI  - Optimization of lipase production on agro-industrial residue medium by 
      Pseudomonas fluorescens (NRLL B-2641) using response surface methodology.
PG  - 64-71
AB  - The aim of our research was to explore the most cost-efficient and optimal medium 
      composition for the production of lipase from Pseudomonas fluorescens (NRLL 
      B-2641) culture grown on sunflower oil cake (SuOC) by applying response surface 
      methodology (RSM). The oil cake was used instead of carbon sources. Peptone, 
      ammonium sulphate and the carbon source (SuOC) were the most important factors as 
      it is obligatory for microbial growth. Subsequently, the optimum values for the 
      carbon source, peptone and ammonium sulphate were found to be 11.10% (w/v), 1.18% 
      (w/v) and 0.83% (w/v), respectively. Experiments carried out under optimum 
      conditions revealed a maximum lipase activity of 10.8 U mL(-1), which was 
      achieved after 48 h of fermentation. The obtained results were finally verified 
      with batch experiments carried out under the optimum conditions evaluated and it 
      was demonstrated that the SuOC from agro-industrial residue as substrates can be 
      used as an inexpensive base (carbon source) for the production of lipase by P. 
      fluorescens (NRLL B-2641).
FAU - Tanyol, Mehtap
AU  - Tanyol M
AD  - Department of Environmental Engineering, Tunceli University , Tunceli , Turkey.
FAU - Uslu, Gulsad
AU  - Uslu G
AD  - Department of Environmental Engineering, Firat University , Elazig , Turkey.
FAU - Yonten, Vahap
AU  - Yonten V
AD  - The Technology of Chemistry, Vocational School, Tunceli University , Tunceli , 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20141216
PL  - England
TA  - Biotechnol Biotechnol Equip
JT  - Biotechnology, biotechnological equipment
JID - 101128940
PMC - PMC4684071
OTO - NOTNLM
OT  - Pseudomonas fluorescens
OT  - RSM
OT  - lipase
OT  - optimization
OT  - sunflower oil cake
EDAT- 2016/01/08 06:00
MHDA- 2016/01/08 06:01
PMCR- 2015/12/18
CRDT- 2016/01/08 06:00
PHST- 2014/06/17 00:00 [received]
PHST- 2014/10/11 00:00 [accepted]
PHST- 2016/01/08 06:00 [entrez]
PHST- 2016/01/08 06:00 [pubmed]
PHST- 2016/01/08 06:01 [medline]
PHST- 2015/12/18 00:00 [pmc-release]
AID - 991635 [pii]
AID - 10.1080/13102818.2014.991635 [doi]
PST - ppublish
SO  - Biotechnol Biotechnol Equip. 2015 Jan 2;29(1):64-71. doi: 
      10.1080/13102818.2014.991635. Epub 2014 Dec 16.