PMID- 26742309
OWN - NLM
STAT- MEDLINE
DCOM- 20160209
LR  - 20191027
IS  - 0255-2922 (Print)
IS  - 0255-2922 (Linking)
VI  - 35
IP  - 6
DP  - 2015 Dec
TI  - Differential expression of immune-related genes between healthy volunteers and 
      type 2 diabetic patients with spleen-deficiency pattern.
PG  - 646-52
AB  - OBJECTIVE: To investigate the clinical differentia tion of spleen-deficiency 
      pattern (SDP), a group of symptoms and signs defined in terms of Traditional 
      Chinese Medicine for its clinical practice. METHODS: Peripheral venous blood (> 3 
      mL) was collected from each of six type 2 diabetes mellitus (T2DM)-SDP patients 
      and six healthy volunteers. After the isolation of peripheral white blood cells 
      (PWBCs), total RNA was extracted, and quality control was performed on all RNA 
      samples. Microarray experiments were conducted using the Agilent human whole 
      genome gene chip, and genes demonstrating differential expression were screened. 
      Bioinformatics analysis was conducted on these genes using several online 
      databases. RESULTS: We screened a total of 175 differentially expressed genes 
      (DEGs), of which 111 (63%) were down-regulated and 64 (37%) were up-regulated in 
      T2DM-SDP patients compared with healthy controls. Among the 175 genes, 158 had 
      biological function annotations: 46 (29%) were directly related to an 
      individual's immune regulation or response, 25 (16%) were associated with 
      substance and energy metabolism of PWBCs which could also indirectly influence 
      immunity, and the remaining 87 (55%) were involved in a variety of PWBC 
      biological processes that might eventually influence the immune function. Kyoto 
      Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the 
      DEGs were predominantly enriched in seven immune-related pathways. Hierarchical 
      cluster analysis identified gene expression patterns that were distinguishable 
      between the two study groups. CONCLUSION: Our results suggest that T2DM-SDP 
      patients experience significant hypoimmunity and/or immune dysfunctions, and 
      possess a specific gene expression profile. These findings offer new insights 
      into SDP and the clinical pattern differentiation of T2DM-SDP.
FAU - Chen, Longhui
AU  - Chen L
FAU - Yang, Zemin
AU  - Yang Z
FAU - Chen, Weiwen
AU  - Chen W
FAU - Li, Ruliu
AU  - Li R
FAU - Lin, Chuanquan
AU  - Lin C
FAU - Guan, Lihua
AU  - Guan L
FAU - Zhu, Zhangzhi
AU  - Zhu Z
FAU - Chen, Ruifang
AU  - Chen R
FAU - Li, Saimei
AU  - Li S
FAU - Zhao, Lingbo
AU  - Zhao L
FAU - Zeng, Jinhao
AU  - Zeng J
FAU - Wang, Jianhua
AU  - Wang J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
SB  - IM
MH  - Adult
MH  - Aged
MH  - Diabetes Mellitus, Type 2/*genetics/immunology/physiopathology
MH  - Female
MH  - Gene Expression Profiling
MH  - Healthy Volunteers
MH  - Humans
MH  - Leukocytes/immunology
MH  - Male
MH  - Middle Aged
MH  - Spleen/immunology/*physiopathology
EDAT- 2016/01/09 06:00
MHDA- 2016/02/10 06:00
CRDT- 2016/01/09 06:00
PHST- 2016/01/09 06:00 [entrez]
PHST- 2016/01/09 06:00 [pubmed]
PHST- 2016/02/10 06:00 [medline]
AID - 10.1016/s0254-6272(15)30154-0 [doi]
PST - ppublish
SO  - J Tradit Chin Med. 2015 Dec;35(6):646-52. doi: 10.1016/s0254-6272(15)30154-0.