PMID- 26742313
OWN - NLM
STAT- MEDLINE
DCOM- 20160209
LR  - 20191027
IS  - 0255-2922 (Print)
IS  - 0255-2922 (Linking)
VI  - 35
IP  - 6
DP  - 2015 Dec
TI  - Neuroprotective effect of ethyl acetate extract from gastrodia elata against 
      transient focal cerebral ischemia in rats induced by middle cerebral artery 
      occlusion.
PG  - 671-8
AB  - OBJECTIVE: To investigate the protective effect of neuroprotection against 
      transient focal cerebral ischemia of the extract from Tianma (Rhizoma Gastrodiae) 
      and the possible mechanisms underlying the action. METHODS: Cerebral 
      ischemia-reperfusion injury was induced through middle cerebral artery occlusion 
      (MCAO). Adult male Sprague-Dawley rats were randomly divided into four groups: 
      sham-operated, ischemia-reperfusion model, 102.6 mg/kg extract treated and 11.4 
      mg/kg extract treated groups. The extract was prepared from gastrodia elata with 
      ethyl acetate. The effect of the extract tested on rat neurological deficits and 
      Cerebral index, cerebral infarct volume, brain injury, terminal dexynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) and B-cell lymphoma-2 (Bcl-2) 
      positive cells. RESULTS: The extract was able to reduce neurological scores, 
      cerebral index and cerebral infarction rate. The brain injury was also relieved 
      by the extract. The results of immunofluorescence staining analysis indicated 
      that the extract increased the expression of Bcl-2 and reduced TUNEL-positive 
      cells significantly in the extract treated groups. CONCLUSION: These results 
      suggested that the extract relieved ischemic injury induced by transient focal 
      cerebral ischemia in rats, and this neuroprotective effect might be partially due 
      to the attenuated apoptosis pathway.
FAU - Duan, Xiaohua
AU  - Duan X
FAU - Wang, Weili
AU  - Wang W
FAU - Liu, Xiaoqing
AU  - Liu X
FAU - Yan, Hanwen
AU  - Yan H
FAU - Dai, Rong
AU  - Dai R
FAU - Lin, Qing
AU  - Lin Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cerebral Arteries/drug effects/metabolism
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Gastrodia/*chemistry
MH  - Humans
MH  - Infarction, Middle Cerebral 
      Artery/genetics/metabolism/physiopathology/*prevention & control
MH  - Male
MH  - Neuroprotective Agents/*administration & dosage
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2016/01/09 06:00
MHDA- 2016/02/10 06:00
CRDT- 2016/01/09 06:00
PHST- 2016/01/09 06:00 [entrez]
PHST- 2016/01/09 06:00 [pubmed]
PHST- 2016/02/10 06:00 [medline]
AID - 10.1016/s0254-6272(15)30158-8 [doi]
PST - ppublish
SO  - J Tradit Chin Med. 2015 Dec;35(6):671-8. doi: 10.1016/s0254-6272(15)30158-8.