PMID- 26742774
OWN - NLM
STAT- MEDLINE
DCOM- 20161109
LR  - 20191210
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 213
DP  - 2016 Feb 2
TI  - Propagation of field highly pathogenic porcine reproductive and respiratory 
      syndrome virus in MARC-145 cells is promoted by cell apoptosis.
PG  - 322-331
LID - S0168-1702(15)30192-1 [pii]
LID - 10.1016/j.virusres.2015.12.023 [doi]
AB  - Infection of porcine reproductive and respiratory syndrome virus (PRRSV) induces 
      cell apoptosis both in vivo and in vitro. However, the correlation between host 
      cell apoptosis and PRRSV replication is unclear. Here, the promotion of PRRSV 
      propagation by cell apoptosis in MARC-145 cells was reported. The observation on 
      propagation of field highly pathogenic PRRSV (HP-PRRSV) in MARC-145 cells showed 
      that infection of overgrown MARC-145 cells obviously elevated virus production 
      and cell apoptosis was triggered in these cells before virus inoculation. The 
      investigation on propagation of field HP-PRRSV in apoptosis induced MARC-145 
      cells displayed that induction of apoptosis further increased the virus 
      production and a vigorous viral RNA replication accompanied by fast virus release 
      in these cells was detected in the initial 24h post infection. In addition, when 
      field HP-PRRSV was serially passed in drug-treated MARC-145 cells, the progeny 
      viruses kept a stable viral titer and infectivity to its native target cells in 
      the tested generations. In summary, these findings demonstrated that apoptotic 
      MARC-145 cells were more susceptible to field HP-PRRSV and propagation of the 
      virus was promoted by effective replication and cell-to-cell transmission of the 
      virus in these cells.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Ge, Mengyun
AU  - Ge M
AD  - Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu 610064, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu 610064, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu 610064, China.
FAU - Liu, Tao
AU  - Liu T
AD  - Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu 610064, China.
FAU - Zeng, Fanya
AU  - Zeng F
AD  - Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu 610064, China. Electronic 
      address: zengfanya@scu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151229
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Epithelial Cells/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*growth & development
MH  - *Virus Replication
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell-to-cell transmission
OT  - Field HP-PRRSV
OT  - Infectious cell cluster
OT  - MARC-145 cells
EDAT- 2016/01/09 06:00
MHDA- 2016/11/10 06:00
CRDT- 2016/01/09 06:00
PHST- 2015/09/01 00:00 [received]
PHST- 2015/12/22 00:00 [revised]
PHST- 2015/12/23 00:00 [accepted]
PHST- 2016/01/09 06:00 [entrez]
PHST- 2016/01/09 06:00 [pubmed]
PHST- 2016/11/10 06:00 [medline]
AID - S0168-1702(15)30192-1 [pii]
AID - 10.1016/j.virusres.2015.12.023 [doi]
PST - ppublish
SO  - Virus Res. 2016 Feb 2;213:322-331. doi: 10.1016/j.virusres.2015.12.023. Epub 2015 
      Dec 29.