PMID- 26743516
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20181113
IS  - 1432-0886 (Electronic)
IS  - 0009-5915 (Linking)
VI  - 125
IP  - 4
DP  - 2016 Sep
TI  - The cryptic Y-autosome translocation in the small Indian mongoose, Herpestes 
      auropunctatus, revealed by molecular cytogenetic approaches.
PG  - 807-15
LID - 10.1007/s00412-015-0572-3 [doi]
AB  - In initial studies of the eutherian small Indian mongoose (Herpestes 
      auropunctatus), the Y chromosome could not be identified in somatic cells. The 
      male chromosome number is uniquely odd, 2n = 35, whereas that of females is 
      2n = 36. Previous reports indicated that this unique karyotype resulted from a 
      translocation of the ancestral Y chromosome to an autosome. However, it has been 
      difficult to identify the chromosomes that harbor the translocated Y chromosomal 
      segment because it is an extremely small euchromatic region. Using a Southern 
      blot analysis, we detected four conserved Y-linked genes, SRY, EIF2S3Y, KDM5D, 
      and ZFY, in the male genome. We cloned homologues of these genes and determined 
      their sequences, which showed high homology to genes in two carnivore species, 
      cat and dog. To unambiguously identify the Y-bearing autosome, we performed 
      immunostaining of pachytene spermatocytes using antibodies against SYCP3, gammaH2AX, 
      and the centromere. We observed trivalent chromosomes, and the associations 
      between the distal ends of the chromosomes were consistent with those of Y and X1 
      chromosomes. The centromere of the Y chromosome was located on the ancestral Y 
      chromosomal segment. We mapped the complementary DNA (cDNA) clones of these genes 
      to the male chromosomes using fluorescence in situ hybridization (FISH), and the 
      linear localization of all genes was confirmed by two-colored FISH. These 
      Y-linked genes were localized to the proximal region of the long arm of a single 
      telomeric chromosome, and we successfully identified the chromosome harboring the 
      ancestral Y chromosomal segment.
FAU - Murata, Chie
AU  - Murata C
AD  - Department of Human Genetics, Institute of Biomedical Sciences, Tokushima 
      University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan.
FAU - Sawaya, Hirohito
AU  - Sawaya H
AD  - Graduate School of Life Science, Hokkaido University, North 10, West 8, Kita-ku, 
      Sapporo, 060-0810, Japan.
FAU - Nakata, Katsushi
AU  - Nakata K
AD  - Yambaru Wildlife Conservation Center, Ministry of the Environment, 263-1 Hiji, 
      Kunigami-son, Kunigami-gun, Okinawa, 905-1413, Japan.
FAU - Yamada, Fumio
AU  - Yamada F
AD  - Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 
      305-8687, Japan.
FAU - Imoto, Issei
AU  - Imoto I
AD  - Department of Human Genetics, Institute of Biomedical Sciences, Tokushima 
      University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan.
FAU - Kuroiwa, Asato
AU  - Kuroiwa A
AD  - Laboratory of Animal Cytogenetics, Faculty of Science, Hokkaido University, Kita 
      10 Nishi 8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan. 
      asatok@sci.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20160107
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Genetic Markers)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Sex-Determining Region Y Protein)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.11.- (Histone Demethylases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cats
MH  - Cells, Cultured
MH  - Centromere/physiology
MH  - Cloning, Molecular
MH  - Dogs
MH  - Genes, Y-Linked/*genetics
MH  - Genetic Markers/*genetics
MH  - Herpestidae/*genetics
MH  - Histone Demethylases/genetics
MH  - In Situ Hybridization, Fluorescence/*veterinary
MH  - *Karyotype
MH  - Kruppel-Like Transcription Factors/genetics
MH  - Male
MH  - Sequence Analysis, DNA
MH  - Sex-Determining Region Y Protein/genetics
MH  - Transcription Factors/genetics
MH  - Translocation, Genetic
MH  - Y Chromosome/*genetics
OTO - NOTNLM
OT  - Chromosome evolution
OT  - Fluorescence in situ hybridization
OT  - Meiosis
OT  - Y chromosome
EDAT- 2016/01/09 06:00
MHDA- 2017/03/28 06:00
CRDT- 2016/01/09 06:00
PHST- 2015/07/05 00:00 [received]
PHST- 2015/12/28 00:00 [accepted]
PHST- 2015/12/16 00:00 [revised]
PHST- 2016/01/09 06:00 [entrez]
PHST- 2016/01/09 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
AID - 10.1007/s00412-015-0572-3 [pii]
AID - 10.1007/s00412-015-0572-3 [doi]
PST - ppublish
SO  - Chromosoma. 2016 Sep;125(4):807-15. doi: 10.1007/s00412-015-0572-3. Epub 2016 Jan 
      7.