PMID- 26746166
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20161230
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 11
IP  - 2
DP  - 2016 Jun 8
TI  - Evaluation of biomolecular distributions in rat brain tissues by means of 
      ToF-SIMS using a continuous beam of Ar clusters.
PG  - 02A307
LID - 10.1116/1.4939251 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides detailed 
      chemical structure information and high spatial resolution images. Therefore, 
      ToF-SIMS is useful for studying biological phenomena such as ischemia. In this 
      study, in order to evaluate cerebral microinfarction, the distribution of 
      biomolecules generated by ischemia was measured with ToF-SIMS. ToF-SIMS data sets 
      were analyzed by means of multivariate analysis for interpreting complex samples 
      containing unknown information and to obtain biomolecular mapping indicated by 
      fragment ions from the target biomolecules. Using conventional ToF-SIMS (primary 
      ion source: Bi cluster ion), it is difficult to detect secondary ions beyond 
      approximately 1000 u. Moreover, the intensity of secondary ions related to 
      biomolecules is not always high enough for imaging because of low concentration 
      even if the masses are lower than 1000 u. However, for the observation of 
      biomolecular distributions in tissues, it is important to detect low amounts of 
      biological molecules from a particular area of tissue. Rat brain tissue samples 
      were measured with ToF-SIMS (J105, Ionoptika, Ltd., Chandlers Ford, UK), using a 
      continuous beam of Ar clusters as a primary ion source. ToF-SIMS with Ar clusters 
      efficiently detects secondary ions related to biomolecules and larger molecules. 
      Molecules detected by ToF-SIMS were examined by analyzing ToF-SIMS data using 
      multivariate analysis. Microspheres (45 mum diameter) were injected into the rat 
      unilateral internal carotid artery (MS rat) to cause cerebral microinfarction. 
      The rat brain was sliced and then measured with ToF-SIMS. The brain samples of a 
      normal rat and the MS rat were examined to find specific secondary ions related 
      to important biomolecules, and then the difference between them was investigated. 
      Finally, specific secondary ions were found around vessels incorporating 
      microspheres in the MS rat. The results suggest that important biomolecules 
      related to cerebral microinfarction can be detected by ToF-SIMS.
FAU - Nakano, Shusuke
AU  - Nakano S
AD  - Department of Material and Life science, Seikei University, 3-3-1, 
      Kichijyoji-kitamachi, Musashino-shi, Tokyo 180-8633, Japan.
FAU - Yokoyama, Yuta
AU  - Yokoyama Y
AD  - Department of Material and Life science, Seikei University, 3-3-1, 
      Kichijyoji-kitamachi, Musashino-shi, Tokyo 180-8633, Japan.
FAU - Aoyagi, Satoka
AU  - Aoyagi S
AD  - Department of Material and Life science, Seikei University, 3-3-1, 
      Kichijyoji-kitamachi, Musashino-shi, Tokyo 180-8633, Japan.
FAU - Himi, Naoyuki
AU  - Himi N
AD  - Department of Physiology 2, Kawasaki Medical School, 577 Matsushima, 
      Kurashiki-shi, Okayama 701-0192, Japan.
FAU - Fletcher, John S
AU  - Fletcher JS
AD  - Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, SE-412 96, 
      Sweden.
FAU - Lockyer, Nicholas P
AU  - Lockyer NP
AD  - School of Chemistry, The University of Manchester, Manchester, M13 9PL, UK.
FAU - Henderson, Alex
AU  - Henderson A
AD  - School of Chemical Engineering and Analytical Science, The University of 
      Manchester, Manchester, M13 9PL, UK.
FAU - Vickerman, John C
AU  - Vickerman JC
AD  - School of Chemical Engineering and Analytical Science, The University of 
      Manchester, Manchester, M13 9PL, UK.
LA  - eng
PT  - Journal Article
DEP - 20160608
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 67XQY1V3KH (Argon)
SB  - IM
MH  - Animals
MH  - Argon
MH  - *Brain Chemistry
MH  - Brain Ischemia/*pathology
MH  - Disease Models, Animal
MH  - Rats
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2016/01/10 06:00
MHDA- 2016/10/14 06:00
CRDT- 2016/01/10 06:00
PHST- 2016/01/10 06:00 [entrez]
PHST- 2016/01/10 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10.1116/1.4939251 [doi]
PST - epublish
SO  - Biointerphases. 2016 Jun 8;11(2):02A307. doi: 10.1116/1.4939251.