PMID- 26748718
OWN - NLM
STAT- MEDLINE
DCOM- 20160531
LR  - 20170915
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Linking)
VI  - 164
IP  - 1-2
DP  - 2016 Jan 14
TI  - Structure of Human DROSHA.
PG  - 81-90
LID - S0092-8674(15)01643-8 [pii]
LID - 10.1016/j.cell.2015.12.019 [doi]
AB  - MicroRNA maturation is initiated by RNase III DROSHA that cleaves the stem loop 
      of primary microRNA. DROSHA functions together with its cofactor DGCR8 in a 
      heterotrimeric complex known as Microprocessor. Here, we report the X-ray 
      structure of DROSHA in complex with the C-terminal helix of DGCR8. We find that 
      DROSHA contains two DGCR8-binding sites, one on each RNase III domain (RIIID), 
      which mediate the assembly of Microprocessor. The overall structure of DROSHA is 
      surprisingly similar to that of Dicer despite no sequence homology apart from the 
      C-terminal part, suggesting that DROSHA may have evolved from a Dicer homolog. 
      DROSHA exhibits unique features, including non-canonical zinc-finger motifs, a 
      long insertion in the first RIIID, and the kinked link between Connector helix 
      and RIIID, which explains the 11-bp-measuring "ruler" activity of DROSHA. Our 
      study implicates the evolutionary origin of DROSHA and elucidates the molecular 
      basis of Microprocessor assembly and primary microRNA processing.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Kwon, S Chul
AU  - Kwon SC
AD  - Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School 
      of Biological Sciences, Seoul National University, Seoul 08826, Korea.
FAU - Nguyen, Tuan Anh
AU  - Nguyen TA
AD  - Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School 
      of Biological Sciences, Seoul National University, Seoul 08826, Korea.
FAU - Choi, Yeon-Gil
AU  - Choi YG
AD  - Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School 
      of Biological Sciences, Seoul National University, Seoul 08826, Korea.
FAU - Jo, Myung Hyun
AU  - Jo MH
AD  - Department of Physics and Astronomy, Seoul National University, Seoul 08826, 
      Korea; National Center for Creative Research Initiatives, Seoul National 
      University, Seoul 08826, Korea; Institute of Applied Physics, Seoul National 
      University, Seoul 08826, Korea.
FAU - Hohng, Sungchul
AU  - Hohng S
AD  - Department of Physics and Astronomy, Seoul National University, Seoul 08826, 
      Korea; National Center for Creative Research Initiatives, Seoul National 
      University, Seoul 08826, Korea; Institute of Applied Physics, Seoul National 
      University, Seoul 08826, Korea; Department of Biophysics and Chemical Biology, 
      Seoul National University, Seoul 08826, Korea.
FAU - Kim, V Narry
AU  - Kim VN
AD  - Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School 
      of Biological Sciences, Seoul National University, Seoul 08826, Korea. Electronic 
      address: narrykim@snu.ac.kr.
FAU - Woo, Jae-Sung
AU  - Woo JS
AD  - Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School 
      of Biological Sciences, Seoul National University, Seoul 08826, Korea. Electronic 
      address: jaesungwoo@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151231
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DGCR8 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.1.26.3 (DICER1 protein, human)
RN  - EC 3.1.26.3 (DROSHA protein, human)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
CIN - Cell Res. 2016 May;26(5):511-2. PMID: 27125999
MH  - Amino Acid Sequence
MH  - Crystallography, X-Ray
MH  - DEAD-box RNA Helicases/chemistry/metabolism
MH  - Evolution, Molecular
MH  - Humans
MH  - MicroRNAs/chemistry/*metabolism
MH  - Models, Chemical
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Protein Folding
MH  - Protein Structure, Tertiary
MH  - *RNA Processing, Post-Transcriptional
MH  - RNA-Binding Proteins/metabolism
MH  - Ribonuclease III/*chemistry/genetics/metabolism
MH  - Sequence Alignment
MH  - Structural Homology, Protein
EDAT- 2016/01/11 06:00
MHDA- 2016/06/01 06:00
CRDT- 2016/01/11 06:00
PHST- 2015/11/05 00:00 [received]
PHST- 2015/11/28 00:00 [revised]
PHST- 2015/12/07 00:00 [accepted]
PHST- 2016/01/11 06:00 [entrez]
PHST- 2016/01/11 06:00 [pubmed]
PHST- 2016/06/01 06:00 [medline]
AID - S0092-8674(15)01643-8 [pii]
AID - 10.1016/j.cell.2015.12.019 [doi]
PST - ppublish
SO  - Cell. 2016 Jan 14;164(1-2):81-90. doi: 10.1016/j.cell.2015.12.019. Epub 2015 Dec 
      31.