PMID- 26749227 OWN - NLM STAT- MEDLINE DCOM- 20170113 LR - 20191210 IS - 1096-9101 (Electronic) IS - 0196-8092 (Linking) VI - 48 IP - 4 DP - 2016 Apr TI - The antibacterial effect of sinoporphyrin sodium photodynamic therapy on Staphylococcus aureus planktonic and biofilm cultures. PG - 400-8 LID - 10.1002/lsm.22468 [doi] AB - BACKGROUND AND OBJECTIVES: Staphylococcus aureus (S. aureus) are important causes of nosocomial and medical-device-related infections. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria. Sinoporphyrin sodium (DVDMS) is a newly identified photosensitizer and has high photo-sensitivity when used in PDT. This study aims to evaluate the antibacterial effect of DVDMS mediated PDT on S. aureus planktonic and biofilm cultures. STUDY DESIGN/MATERIALS AND METHODS: The uptake of DVDMS in S. aureus was evaluated according to photometry after alkali lysis. Then bacteria were incubated with DVDMS and exposed to light treatment. After PDT treatment, counting colony-forming units (CFU) was applied to estimate the bactericidal effect. Intracellular reactive oxygen species (ROS) production was detected by flow cytometry. Scanning electron microscope (SEM) was performed to assess the disruption of biofilm. RESULTS: With the incubation time increased, the relative fluorescence intensity of DVDMS in bacteria increased and reached peak at 75 minutes. DVDMS alone did not produce significant toxicity compared with the untreated group, while, remarkable survival decrease was observed in PDT groups in a dose-dependent manner. More than 90% of the bacteria were effectively killed by the combined treatment of 2 microM DVDMS with 10 J/cm2 light irradiation, and 4-log reduction in CFU was observed after 5 microM DVDMS treatment followed by 100 J/cm2 light irradiation. Intracellular ROS level was significantly enhanced after PDT treatment. The disruption of biofilm was confirmed by SEM, suggesting DVDMS-PDT effectively damaged the biofilm. CONCLUSION: These results indicate DVDMS-PDT presents significant bactericidal activity. CI - (c) 2016 Wiley Periodicals, Inc. FAU - Mai, Bingjie AU - Mai B AD - Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China. FAU - Wang, Xiaobing AU - Wang X AD - Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China. FAU - Liu, Quanhong AU - Liu Q AD - Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China. FAU - Leung, Albert Wingnang AU - Leung AW AD - School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China. FAU - Wang, Xinliang AU - Wang X AD - Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China. FAU - Xu, Chuanshan AU - Xu C AD - School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China. FAU - Wang, Pan AU - Wang P AD - Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China. AD - School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China. LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160108 PL - United States TA - Lasers Surg Med JT - Lasers in surgery and medicine JID - 8007168 RN - 0 (Anti-Bacterial Agents) RN - 0 (Biomarkers) RN - 0 (Photosensitizing Agents) RN - 0 (Porphyrins) RN - 0 (Reactive Oxygen Species) RN - 0 (sinoporphyrin sodium) SB - IM MH - Anti-Bacterial Agents/*pharmacology MH - Biofilms/*drug effects/growth & development MH - Biomarkers/metabolism MH - Colony Count, Microbial MH - Microscopy, Electron, Scanning MH - Photochemotherapy/*methods MH - Photosensitizing Agents/*pharmacology MH - Plankton/*drug effects/physiology MH - Porphyrins/*pharmacology MH - Reactive Oxygen Species/metabolism MH - Staphylococcus aureus/*drug effects/physiology OTO - NOTNLM OT - antibacterial efficacy OT - antimicrobial photodynamic inactivation OT - photodynamic inactivation EDAT- 2016/01/11 06:00 MHDA- 2017/01/14 06:00 CRDT- 2016/01/11 06:00 PHST- 2015/12/23 00:00 [accepted] PHST- 2016/01/11 06:00 [entrez] PHST- 2016/01/11 06:00 [pubmed] PHST- 2017/01/14 06:00 [medline] AID - 10.1002/lsm.22468 [doi] PST - ppublish SO - Lasers Surg Med. 2016 Apr;48(4):400-8. doi: 10.1002/lsm.22468. Epub 2016 Jan 8.