PMID- 26750016
OWN - NLM
STAT- MEDLINE
DCOM- 20160712
LR  - 20181202
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 29
IP  - 9
DP  - 2015 Sep
TI  - [EXPERIMENTAL STUDY ON HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS-ALGINATE WOUND 
      DRESSING].
PG  - 1137-43
AB  - OBJECTIVE: To observe the growth characteristics of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) cultured on the alginate gel scaffolds and to 
      explore the feasibility of hUCMSCs-alginate dressing for wound healing. METHODS: 
      hUCMSCs were separated from human umbilical cords and cultured in vitro. After 
      the 4th passage cells were co-cultured with alginate gel (experimental group), 
      the cell growth characteristics were observed under the inverted phase contrast 
      microscope. Vascular endothelial growth factor (VEGF) content was measured and 
      the number of cells was counted at 0, 3, 6, and 9 days after culture; and the 
      cell migration capacity was observed. The hUCMSCs were cultured without alginated 
      gel as control. The model of full-thickness skin defects was established in 32 
      8-week-old Balb/c male mice and they were randomly divided into 4 groups (n=8): 
      wounds were covered with hUCMSCs-alginate gel compound (MSC-gel group), cell 
      supernatants-alginate gel compound (CS-gel group), 10% FBS-alginate gel compound 
      (FBS-gel group), and 0.01 mol/L PBS-alginate compound (PBS-gel group), 
      respectively. Wound healing rates at 5, 10, and 15 days were observed and 
      calculated; and the wound tissues were harvested for histological and 
      immunohistochemical staining to assess new skin conditions at 15 days after 
      operation. RESULTS: hUCMSCs grew well with grape-like proliferation on the 
      alginate gel, but no cell migration was observed at 7 days after cultivation. 
      VEGF expression and cell number in experimental group were significantly less 
      than those in control group at 3 days (P<0.05); then they gradually increased, 
      and VEGF expression and cell number were significantly more than those in control 
      group at 9 days (P<0.05). The wound healing rates of MSC-gel and CS-gel groups 
      were significantly higher than those of FBS-gel and PBS-gel groups at 5, 10, and 
      15 days (P<0.05). The squamous epithelium, fibroblasts, sebaceous glands, 
      capillaries and VEGF expression of the new skin in MSC-gel and CS-gel groups were 
      significantly more than FBS-gel and PBS-gel groups (P<0.05). But there was no 
      significance between MSC-gel and CS-gel groups (P>0.05). CONCLUSION: hUCMSCs can 
      continuously express VEGF in alginate gel, which is necessary for wound healing. 
      The hUCMSCs-alginate compound is probably a good wound dressing.
FAU - Wang, Song
AU  - Wang S
FAU - Su, Meilan
AU  - Su M
FAU - Yang, Huachao
AU  - Yang H
FAU - Long, Gang
AU  - Long G
FAU - Tang, Zhenrui
AU  - Tang Z
FAU - Huang, Wen
AU  - Huang W
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Alginates)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 8A5D83Q4RW (Glucuronic Acid)
SB  - IM
MH  - Alginates
MH  - Animals
MH  - *Bandages
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Fibroblasts
MH  - Glucuronic Acid
MH  - Hexuronic Acids
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - *Umbilical Cord
MH  - Vascular Endothelial Growth Factor A
MH  - *Wound Healing
EDAT- 2016/01/12 06:00
MHDA- 2016/07/13 06:00
CRDT- 2016/01/12 06:00
PHST- 2016/01/12 06:00 [entrez]
PHST- 2016/01/12 06:00 [pubmed]
PHST- 2016/07/13 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2015 Sep;29(9):1137-43.