PMID- 26751994
OWN - NLM
STAT- MEDLINE
DCOM- 20161103
LR  - 20161230
IS  - 1536-4798 (Electronic)
IS  - 0277-3740 (Linking)
VI  - 35
IP  - 4
DP  - 2016 Apr
TI  - Effect of Topically Applied Azithromycin on Corneal Epithelial and Endothelial 
      Apoptosis in a Rat Model of Corneal Alkali Burn.
PG  - 543-9
LID - 10.1097/ICO.0000000000000730 [doi]
AB  - PURPOSE: To investigate the antiapoptotic effect of topically administered 
      azithromycin (AZM) on corneal epithelial and endothelial cells in a rat model of 
      corneal alkali burn. METHODS: Twenty-four Wistar albino rats were divided into 4 
      equal groups as pseudovehicle (group 1), control (group 2), alkali burned (group 
      3), and treatment (group 4) groups. Alkali injury was induced only in the right 
      corneas of rats belonging to groups 3 and 4 using 1N NaOH. The rats in group 3 
      and the rats in group 4 were respectively treated either with an artificial tear 
      gel or with 1.5% AZM eye drops for 5 days. At the fifth day of the experiment, 
      the apoptosis in the corneal epithelium and endothelium of all rats was assessed 
      using a terminal dUTP nick-end labeling (TUNEL) assay. In addition, tumor 
      necrosis factor-alpha (TNF-alpha) density in the corneal epithelium was measured in 
      all rats. RESULTS: The mean numbers of TUNEL+ cells in the corneal epithelium and 
      endothelium of rats in group 3 were 117.1 +/- 23.8 and 34.6.+/- 11.3, respectively, 
      whereas in group 4, they were 75.8 +/- 15.7 and 14.7 +/- 3.5, respectively. Also the 
      mean TNF-alpha densities in the corneal epithelium in group 3 and group 4 were 2.65 +/- 
      1.3 and 1.65 +/- 1.1, respectively. There was a significant decrease in the mean 
      number of TUNEL+ cells in the corneal epithelium and endothelium and in the mean 
      TNF-alpha density in the corneal epithelium of rats in group 4, when compared with 
      group 3. CONCLUSIONS: Topically applied AZM can decrease TNF-alpha-induced apoptosis 
      in corneal alkali burn.
FAU - Arikan, Sedat
AU  - Arikan S
AD  - *Department of Ophthalmology, School of Medicine, Canakkale Onsekiz Mart 
      University, Canakkale, Turkey; daggerDepartment of Histology and Embryology, School of 
      Medicine, Trakya University, Edirne, Turkey; double daggerDepartment of Family Medicine, 
      School of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey; 
       section signCanakkale Onsekiz Mart University Experimental Research Center, Experimental 
      Research Application and Research Center, Canakkale Onsekiz Mart University, 
      Canakkale, Turkey; and  paragraph signDepartment of Biochemistry, School of Medicine, Canakkale 
      Onsekiz Mart University, Canakkale, Turkey.
FAU - Karaca, Turan
AU  - Karaca T
FAU - Ertekin, Yusuf Haydar
AU  - Ertekin YH
FAU - Comez, Arzu Taskiran
AU  - Comez AT
FAU - Ersan, Ismail
AU  - Ersan I
FAU - Demirtas, Selim
AU  - Demirtas S
FAU - Elmas, Sait
AU  - Elmas S
FAU - Tufan, Hasan Ali
AU  - Tufan HA
FAU - Turkon, Hakan
AU  - Turkon H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 55X04QC32I (Sodium Hydroxide)
RN  - 83905-01-5 (Azithromycin)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Anti-Bacterial Agents/*administration & dosage
MH  - Apoptosis/*drug effects
MH  - Azithromycin/*administration & dosage
MH  - Burns, Chemical/metabolism/*pathology
MH  - Disease Models, Animal
MH  - Endothelium, Corneal/metabolism/*pathology
MH  - Epithelium, Corneal/metabolism/*pathology
MH  - Eye Burns/*chemically induced
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium Hydroxide
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2016/01/12 06:00
MHDA- 2016/11/04 06:00
CRDT- 2016/01/12 06:00
PHST- 2016/01/12 06:00 [entrez]
PHST- 2016/01/12 06:00 [pubmed]
PHST- 2016/11/04 06:00 [medline]
AID - 10.1097/ICO.0000000000000730 [doi]
PST - ppublish
SO  - Cornea. 2016 Apr;35(4):543-9. doi: 10.1097/ICO.0000000000000730.