PMID- 26752309
OWN - NLM
STAT- MEDLINE
DCOM- 20170728
LR  - 20181202
IS  - 1552-8618 (Electronic)
IS  - 0730-7268 (Linking)
VI  - 35
IP  - 7
DP  - 2016 Jul
TI  - Transfer of benzo[a]pyrene from microplastics to Artemia nauplii and further to 
      zebrafish via a trophic food web experiment: CYP1A induction and visual tracking 
      of persistent organic pollutants.
PG  - 1656-66
LID - 10.1002/etc.3361 [doi]
AB  - The uptake of microplastic particles and the transfer of potential harmful 
      substances along with microplastics has been studied in a variety of organisms, 
      especially invertebrates. However, the potential accumulation of very small 
      microplastic particles along food webs ending with vertebrate models has not been 
      investigated so far. Therefore, a simple artificial food chain with Artemia sp. 
      nauplii and zebrafish (Danio rerio) was established to analyze the transfer of 
      microplastic particles and associated persistent organic pollutants (POPs) 
      between different trophic levels. Very small (1-20 mum) microplastic particles 
      accumulated in Artemia nauplii and were subsequently transferred to fish. Virgin 
      particles not loaded with POPs did not cause any observable physical harm in the 
      intestinal tracts of zebrafish, although parts of the particles were retained 
      within the mucus of intestinal villi and might even have been taken up by 
      epithelial cells. The transfer of associated POPs was tested with the polycyclic 
      aromatic hydrocarbon benzo[a]pyrene and an ethoxyresorufin-O-deethylase (EROD) 
      assay for CYP1A induction in zebrafish liver as well as via fluorescence 
      analyses. Whereas a significant induction in the EROD assay could not be shown, 
      because of high individual variation and low sensitivity regarding substance 
      concentration, the fluorescence tracking of benzo[a]pyrene indicates that 
      food-borne microplastic-associated POPs may actually desorb in the intestine of 
      fish and are thus transferred to the intestinal epithelium and liver. Environ 
      Toxicol Chem 2016;35:1656-1666. (c) 2016 SETAC.
CI  - (c) 2016 SETAC.
FAU - Batel, Annika
AU  - Batel A
AD  - Aquatic Ecology and Toxicology, Center for Organismal Studies, University of 
      Heidelberg, Heidelberg, Germany.
FAU - Linti, Frederic
AU  - Linti F
AD  - Aquatic Ecology and Toxicology, Center for Organismal Studies, University of 
      Heidelberg, Heidelberg, Germany.
FAU - Scherer, Martina
AU  - Scherer M
AD  - Aquatic Ecology and Toxicology, Center for Organismal Studies, University of 
      Heidelberg, Heidelberg, Germany.
FAU - Erdinger, Lothar
AU  - Erdinger L
AD  - Aquatic Ecology and Toxicology, Center for Organismal Studies, University of 
      Heidelberg, Heidelberg, Germany.
FAU - Braunbeck, Thomas
AU  - Braunbeck T
AD  - Aquatic Ecology and Toxicology, Center for Organismal Studies, University of 
      Heidelberg, Heidelberg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20160510
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Artemia/*metabolism
MH  - Benzo(a)pyrene/*analysis/metabolism
MH  - Cytochrome P-450 CYP1A1/*biosynthesis
MH  - Environmental Monitoring/*methods
MH  - Food Chain
MH  - Liver/drug effects/enzymology
MH  - Microsomes, Liver/drug effects/enzymology
MH  - Models, Theoretical
MH  - Optical Imaging
MH  - Particle Size
MH  - Water Pollutants, Chemical/*analysis/metabolism
MH  - Zebrafish/*metabolism
OTO - NOTNLM
OT  - Benzo[a]pyrene
OT  - CYP1A induction
OT  - Food web
OT  - Microplastics
OT  - Zebrafish
EDAT- 2016/01/12 06:00
MHDA- 2017/07/29 06:00
CRDT- 2016/01/12 06:00
PHST- 2015/07/01 00:00 [received]
PHST- 2015/09/14 00:00 [revised]
PHST- 2015/12/24 00:00 [accepted]
PHST- 2016/01/12 06:00 [entrez]
PHST- 2016/01/12 06:00 [pubmed]
PHST- 2017/07/29 06:00 [medline]
AID - 10.1002/etc.3361 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2016 Jul;35(7):1656-66. doi: 10.1002/etc.3361. Epub 2016 
      May 10.