PMID- 26752934
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160112
LR  - 20220310
IS  - 1000-9604 (Print)
IS  - 1993-0631 (Electronic)
IS  - 1000-9604 (Linking)
VI  - 27
IP  - 6
DP  - 2015 Dec
TI  - Immune responses of dendritic cells combined with tumor-derived autophagosome 
      vaccine on hepatocellular carcinoma.
PG  - 597-603
LID - 10.3978/j.issn.1000-9604.2015.12.07 [doi]
AB  - BACKGROUND: To induce and collect tumor-derived autophagosomes (DRibbles) from 
      tumor cells as an antitumor vaccine by inhibiting the functions of proteasomes 
      and lysosomes. METHODS: Dendritic cells (DCs) generated from peripheral blood 
      mononuclear cell (PBMC) of hepatocellular carcinoma (HCC) patients were 
      cocultured with DRibbles, and then surface molecules of DCs, as well as surface 
      molecules on DCs, were determined by flow cytometry. Meanwhile, immune responses 
      of the DCs-DRibbles were examined by mixed lymphocyte reactions. RESULTS: 
      DRibbles significantly induced the expression of CD80, CD83, CD86 and HLA-DR on 
      DCs. The enzyme-linked immunosorbnent assay (ELISA) showed that IFN-gamma levels 
      after vaccination increased than before in most patients, but CD8+ proportion of 
      PBMC increased only in nine patients. Higher levels of IFN-gamma were detected in the 
      CD8+ cells than CD4+ T cells. These results suggested that DCs-DRibbles vaccine 
      could induce antigen-specific cellular immune response on HCC and could prime 
      strong CD8+ T cell responses, supporting it as a tumor vaccine candidate. 
      CONCLUSIONS: Our results demonstrate that HCC/DRibbles-pulsed DCs immunotherapy 
      might be deployed as an effective antitumor vaccine for HCC immunotherapy in 
      clinical trials.
FAU - Yi, Yongxiang
AU  - Yi Y
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Han, Jianbo
AU  - Han J
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Zhao, Liang
AU  - Zhao L
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Wang, Chunying
AU  - Wang C
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Fang, Yuan
AU  - Fang Y
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Wei, Qiang
AU  - Wei Q
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Hu, Liang
AU  - Hu L
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Liu, Junmao
AU  - Liu J
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Zhang, Yufeng
AU  - Zhang Y
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
FAU - Wang, Lili
AU  - Wang L
AD  - 1 Department of Hepatobiliary Surgery, the Second Hospital of Nanjing, Affiliated 
      to Southeast University, Nanjing 210003, China ; 2 Department of Hepatic Internal 
      Medicine, Xuzhou Hospital for Infectious Diseases, Xuzhou 221004, China ; 3 
      Department of Immunotherapy, 4 Department of Interventional Ultrasound, the 
      Second Hospital of Nanjing, Affiliated to Southeast University, Nanjing 210003, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Cancer Res
JT  - Chinese journal of cancer research = Chung-kuo yen cheng yen chiu
JID - 9315242
PMC - PMC4697098
OTO - NOTNLM
OT  - Autophagy
OT  - antitumor immunity
OT  - dendritic cells (DCs)
OT  - hepatocellular carcinoma (HCC)
COIS- Conflicts of Interest: The authors have no conflicts of interest to declare.
EDAT- 2016/01/12 06:00
MHDA- 2016/01/12 06:01
PMCR- 2015/12/01
CRDT- 2016/01/12 06:00
PHST- 2016/01/12 06:00 [entrez]
PHST- 2016/01/12 06:00 [pubmed]
PHST- 2016/01/12 06:01 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - cjcr-27-06-597 [pii]
AID - 10.3978/j.issn.1000-9604.2015.12.07 [doi]
PST - ppublish
SO  - Chin J Cancer Res. 2015 Dec;27(6):597-603. doi: 
      10.3978/j.issn.1000-9604.2015.12.07.