PMID- 26753289
OWN - NLM
STAT- MEDLINE
DCOM- 20160122
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 9
DP  - 2015 Sep
TI  - [Determination of 35 antibiotic residues of tetracyclines, sulfonamides, 
      penicillins, macrolides and amphenicols in milk by liquid chromatography-tandem 
      mass spectromtery].
PG  - 995-1001
AB  - A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was 
      established for the simultaneous determination of 35 antibiotic residues of 
      tetracyclines, sulfonamides, penicillins, macrolides and amphenicols in milk. The 
      samples were extracted with alkaline acetonitrile and McIlvaine buffer solution 
      under ultrasonication. The separation of target compounds was performed on an 
      Eclipse XDB-C, column (150 mm x 2.1 mm, 3.5 microm) with gradient elution at a flow 
      rate of 0.25 mL/min, and with an injection volume of 10 microL. The identification 
      and quantification of the compounds were completed by liquid 
      chromatography-tandem mass spectrometry in multiple reaction monitoring ( MRM) 
      mode. The limits of detection were all below 10.0 microg/kg. The average spiked 
      recoveries of the method ranged from 70. 1% to 109. 9% with relative standard 
      deviations (RSDs) of 2.89%-9.99%. After validation, the method was applied to the 
      analysis of antibiotic residues in milk products in China. Fifty samples were 
      screened under the well defined methodology, and the results showed that 
      chloramphenicol, only in one sample, was monitored with the content of 0.48 
      microg/kg. A risk of contamination of milk with chloramphenicol has been determined 
      to exist. Therefore this method is convenient, rapid, sensitive and reliable, and 
      can be successfully applied to the simultaneous detection of the 35 antibiotic 
      residues of tetracyclines, sulfonamides, penicillins, macrolides and amphenicols 
      in milk.
FAU - Wang, Hao
AU  - Wang H
FAU - Zhao, Li
AU  - Zhao L
FAU - Yang, Hongmei
AU  - Yang H
FAU - Pan, Hongyan
AU  - Pan H
FAU - Shi, Hailiang
AU  - Shi H
FAU - Qian, Cong
AU  - Qian C
FAU - Zhang, Shan
AU  - Zhang S
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Macrolides)
RN  - 0 (Penicillins)
RN  - 0 (Sulfanilamides)
RN  - 0 (Sulfonamides)
RN  - 0 (Tetracyclines)
RN  - 21240MF57M (Sulfanilamide)
RN  - 66974FR9Q1 (Chloramphenicol)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - China
MH  - Chloramphenicol
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Macrolides
MH  - Milk/*chemistry
MH  - Penicillins
MH  - Sulfanilamide
MH  - Sulfanilamides
MH  - Sulfonamides
MH  - Tandem Mass Spectrometry
MH  - Tetracyclines
EDAT- 2016/01/13 06:00
MHDA- 2016/01/23 06:00
CRDT- 2016/01/13 06:00
PHST- 2016/01/13 06:00 [entrez]
PHST- 2016/01/13 06:00 [pubmed]
PHST- 2016/01/23 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.04046 [doi]
PST - ppublish
SO  - Se Pu. 2015 Sep;33(9):995-1001. doi: 10.3724/sp.j.1123.2015.04046.