PMID- 26753787
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20161230
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 11
IP  - 2
DP  - 2016 Jun 11
TI  - Mass spectrometric profiling of lipids in intestinal tissue from rats fed cereals 
      processed for medical conditions.
PG  - 02A310
LID - 10.1116/1.4939599 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used for lipid 
      profiling of intestine tissue sections from rats fed specially processed cereals 
      and rats fed ordinary feed as a control. This cereal is known to increase the 
      activity of antisecretory factor in plasma and the exact mechanism for the 
      activation process at the cellular level is unclear. ToF-SIMS has been used to 
      track food induced changes in lipid content in intestinal tissue sections to gain 
      insight into the possible mechanisms involved. Data from 20 intestine sections 
      belonging to four different rats from each group of control and specially 
      processed cereals-fed rats were obtained using the stage scan macroraster with a 
      lateral resolution of 5 mum. Data were subsequently subjected to orthogonal 
      partial least squares discriminant analysis. The data clearly show that changes 
      of certain lipids are induced by the specially processed cereal feed. Scores 
      plots show a well-defined separation between the two groups. The corresponding 
      loading plots reveal that the groups separate mainly due to changes of vitamin E, 
      phosphocholine, and phosphosphingolipid fragments, and that for the c18:2 fatty 
      acid. The observed changes in lipids might give insight into the working 
      mechanisms of antisecretory factor in the body, and this has been successfully 
      used to understand the working mechanism of specially processed cereal-induced 
      antisecretory factor activation in intestine.
FAU - Dowlatshahi Pour, Masoumeh
AU  - Dowlatshahi Pour M
AD  - Department of Chemistry and Chemical Engineering, Chalmers University of 
      Technology, Gothenburg 412 96, Sweden and National Center for Imaging Mass 
      Spectrometry, Gothenburg 412 96, Sweden.
FAU - Jennische, Eva
AU  - Jennische E
AD  - Institute of Biomedicine, Gothenburg 413 90, Sweden.
FAU - Lange, Stefan
AU  - Lange S
AD  - Institute of Biomedicine, Gothenburg 413 90, Sweden.
FAU - Ewing, Andrew G
AU  - Ewing AG
AD  - Department of Chemistry and Chemical Engineering, Chalmers University of 
      Technology, Gothenburg 412 96, Sweden; National Center for Imaging Mass 
      Spectrometry, Gothenburg 412 96, Sweden; and Department of Chemistry and 
      Molecular Biology, University of Gothenburg, Gothenburg 412 96, Sweden.
FAU - Malmberg, Per
AU  - Malmberg P
AD  - Department of Chemistry and Chemical Engineering, Chalmers University of 
      Technology, Gothenburg 412 96, Sweden and National Center for Imaging Mass 
      Spectrometry, Gothenburg 412 96, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20160611
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Lipids)
SB  - IM
MH  - *Animal Feed
MH  - Animals
MH  - Diet/*methods
MH  - *Edible Grain
MH  - Intestines/*chemistry
MH  - Lipids/*analysis
MH  - Rats
MH  - Spectrometry, Mass, Secondary Ion/methods
EDAT- 2016/01/13 06:00
MHDA- 2016/10/14 06:00
CRDT- 2016/01/13 06:00
PHST- 2016/01/13 06:00 [entrez]
PHST- 2016/01/13 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10.1116/1.4939599 [doi]
PST - epublish
SO  - Biointerphases. 2016 Jun 11;11(2):02A310. doi: 10.1116/1.4939599.