PMID- 26755602
OWN - NLM
STAT- MEDLINE
DCOM- 20160617
LR  - 20181113
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 113
IP  - 4
DP  - 2016 Jan 26
TI  - Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph 
      node.
PG  - 1044-9
LID - 10.1073/pnas.1513607113 [doi]
AB  - Dendritic cells (DCs) are antigen-presenting cells specialized for activating T 
      cells to elicit effector T-cell functions. Cross-presenting DCs are a DC subset 
      capable of presenting antigens to CD8(+) T cells and play critical roles in 
      cytotoxic T-cell-mediated immune responses to microorganisms and cancer. Although 
      their importance is known, the spatiotemporal dynamics of cross-presenting DCs in 
      vivo are incompletely understood. Here, we study the T-cell zone in skin-draining 
      lymph nodes (SDLNs) and find it is compartmentalized into regions for CD8(+) 
      T-cell activation by cross-presenting DCs that express the chemokine (C motif) 
      receptor 1 gene, Xcr1 and for CD4(+) T-cell activation by CD11b(+) DCs. 
      Xcr1-expressing DCs in the SDLNs are composed of two different populations: 
      migratory (CD103(hi)) DCs, which immigrate from the skin, and resident (CD8alpha(hi)) 
      DCs, which develop in the nodes. To characterize the dynamic interactions of 
      these distinct DC populations with CD8(+) T cells during their activation in 
      vivo, we developed a photoconvertible reporter mouse strain, which permits us to 
      distinctively visualize the migratory and resident subsets of Xcr1-expressing 
      DCs. After leaving the skin, migratory DCs infiltrated to the deep T-cell zone of 
      the SDLNs over 3 d, which corresponded to their half-life in the SDLNs. 
      Intravital two-photon imaging showed that after soluble antigen immunization, the 
      newly arriving migratory DCs more efficiently form sustained conjugates with 
      antigen-specific CD8(+) T cells than other Xcr1-expressing DCs in the SDLNs. 
      These results offer in vivo evidence for differential contributions of migratory 
      and resident cross-presenting DCs to CD8(+) T-cell activation.
FAU - Kitano, Masahiro
AU  - Kitano M
AD  - Laboratory for Tissue Dynamics, RIKEN Center for Integrative Medical Sciences, 
      Yokohama, Kanagawa 230-0045, Japan; Translational Imaging Center, University of 
      Southern California, Los Angeles, CA 90089;
FAU - Yamazaki, Chihiro
AU  - Yamazaki C
AD  - Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, 
      Yokohama, Kanagawa 230-0045, Japan; Department of Allergy and Immunology, 
      Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; 
      Department of Immunology, Graduate School of Medicine, Dentistry, and 
      Pharmaceutical Sciences, Okayama University, Okayama, Okayama 700-8558, Japan;
FAU - Takumi, Akiko
AU  - Takumi A
AD  - Laboratory for Tissue Dynamics, RIKEN Center for Integrative Medical Sciences, 
      Yokohama, Kanagawa 230-0045, Japan;
FAU - Ikeno, Takashi
AU  - Ikeno T
AD  - Laboratory for Tissue Dynamics, RIKEN Center for Integrative Medical Sciences, 
      Yokohama, Kanagawa 230-0045, Japan; Laboratory of Lymphocyte Differentiation, 
      Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, 
      Japan;
FAU - Hemmi, Hiroaki
AU  - Hemmi H
AD  - Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, 
      Yokohama, Kanagawa 230-0045, Japan; Laboratory for Inflammatory Regulation, RIKEN 
      Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; 
      Laboratory for Immune Regulation, World Premier International Research Center 
      Initiative, Immunology Frontier Research Center, Osaka University, Suita, Osaka 
      565-0871, Japan; Department of Immunology, Institute of Advanced Medicine, 
      Wakayama Medical University, Wakayama, Wakayama 641-8509, Japan;
FAU - Takahashi, Noriko
AU  - Takahashi N
AD  - Laboratory for Tissue Dynamics, RIKEN Center for Integrative Medical Sciences, 
      Yokohama, Kanagawa 230-0045, Japan;
FAU - Shimizu, Kanako
AU  - Shimizu K
AD  - Laboratory for Immunotherapy, RIKEN Center for Integrative Medical Sciences, 
      Yokohama, Kanagawa 230-0045, Japan;
FAU - Fraser, Scott E
AU  - Fraser SE
AD  - Translational Imaging Center, University of Southern California, Los Angeles, CA 
      90089;
FAU - Hoshino, Katsuaki
AU  - Hoshino K
AD  - Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, 
      Yokohama, Kanagawa 230-0045, Japan; Laboratory for Inflammatory Regulation, RIKEN 
      Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; 
      Laboratory for Immune Regulation, World Premier International Research Center 
      Initiative, Immunology Frontier Research Center, Osaka University, Suita, Osaka 
      565-0871, Japan; Department of Immunology, Faculty of Medicine, Kagawa 
      University, Miki, Kagawa 761-0793, Japan;
FAU - Kaisho, Tsuneyasu
AU  - Kaisho T
AD  - Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, 
      Yokohama, Kanagawa 230-0045, Japan; Department of Allergy and Immunology, 
      Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; 
      Laboratory for Inflammatory Regulation, RIKEN Center for Integrative Medical 
      Sciences, Yokohama, Kanagawa 230-0045, Japan; Laboratory for Immune Regulation, 
      World Premier International Research Center Initiative, Immunology Frontier 
      Research Center, Osaka University, Suita, Osaka 565-0871, Japan; Department of 
      Immunology, Institute of Advanced Medicine, Wakayama Medical University, 
      Wakayama, Wakayama 641-8509, Japan; takaharu.okada@riken.jp 
      tkaisho@wakayama-med.ac.jp.
FAU - Okada, Takaharu
AU  - Okada T
AD  - Laboratory for Tissue Dynamics, RIKEN Center for Integrative Medical Sciences, 
      Yokohama, Kanagawa 230-0045, Japan; Precursory Research for Embryonic Science and 
      Technology, Japan Science and Technology Agency, Saitama, Saitama 332-0012, 
      Japan; Graduate School of Medical Life Science, Yokohama City University, 
      Yokohama 230-0045, Japan takaharu.okada@riken.jp tkaisho@wakayama-med.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160111
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, CD)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (XC chemokine receptor 1, mouse)
RN  - 0 (alpha E integrins)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Movement
MH  - *Cross-Priming
MH  - Dendritic Cells/*immunology
MH  - Immunization
MH  - Integrin alpha Chains/analysis
MH  - Lymph Nodes/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Chemokine/analysis/physiology
MH  - Skin/*immunology
PMC - PMC4743831
OTO - NOTNLM
OT  - CD8+ T cell
OT  - cross-presentation
OT  - dendritic cell
OT  - intravital two-photon imaging
OT  - photoconversion
COIS- The authors declare no conflict of interest.
EDAT- 2016/01/13 06:00
MHDA- 2016/06/18 06:00
PMCR- 2016/01/11
CRDT- 2016/01/13 06:00
PHST- 2016/01/13 06:00 [entrez]
PHST- 2016/01/13 06:00 [pubmed]
PHST- 2016/06/18 06:00 [medline]
PHST- 2016/01/11 00:00 [pmc-release]
AID - 1513607113 [pii]
AID - 201513607 [pii]
AID - 10.1073/pnas.1513607113 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2016 Jan 26;113(4):1044-9. doi: 
      10.1073/pnas.1513607113. Epub 2016 Jan 11.