PMID- 26757811
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20181113
IS  - 1940-9818 (Electronic)
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 60
IP  - 1
DP  - 2016 Jan
TI  - A simple, low-cost staining method for rapid-throughput analysis of tumor 
      spheroids.
PG  - 43-6
LID - 10.2144/000114372 [doi]
AB  - Tumor spheroids are becoming an important tool for the investigation of cancer 
      stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods 
      for drug screening of tumor spheroids are needed. Using neurospheres as 
      non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost 
      acridine orange (AO)-based method that allows for rapid analysis of live 
      neurospheres by fluorescence microscopy in a 96-well format. This assay measures 
      the cross-section area of a spheroid, which corresponds to cell viability. Our 
      novel method allows rapid screening of a panel of anti-proliferative drugs to 
      assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.
FAU - Eckerdt, Frank
AU  - Eckerdt F
AD  - Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, 
      Northwestern University Feinberg School of Medicine, Chicago, IL.
FAU - Alvarez, Angel
AU  - Alvarez A
AD  - Department of Neurology, Robert H. Lurie Comprehensive Cancer Center, 
      Northwestern Brain Tumor Institute, Northwestern University Feinberg School of 
      Medicine, Chicago, IL.
FAU - Bell, Jonathan
AU  - Bell J
AD  - Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, 
      Northwestern University Feinberg School of Medicine, Chicago, IL.
FAU - Arvanitis, Constadina
AU  - Arvanitis C
AD  - Center for Advanced Microscopy and Department of Pathology, Northwestern 
      University Feinberg School of Medicine, Chicago, IL.
FAU - Iqbal, Asneha
AU  - Iqbal A
AD  - Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, 
      Northwestern University Feinberg School of Medicine, Chicago, IL.
AD  - Division of Hematology and Oncology, Ann & Robert H. Lurie Children's Hospital of 
      Chicago, Chicago, IL.
FAU - Arslan, Ahmet D
AU  - Arslan AD
AD  - Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, 
      Northwestern University Feinberg School of Medicine, Chicago, IL.
FAU - Hu, Bo
AU  - Hu B
AD  - Department of Neurology, Robert H. Lurie Comprehensive Cancer Center, 
      Northwestern Brain Tumor Institute, Northwestern University Feinberg School of 
      Medicine, Chicago, IL.
FAU - Cheng, Shi-Yuan
AU  - Cheng SY
AD  - Department of Neurology, Robert H. Lurie Comprehensive Cancer Center, 
      Northwestern Brain Tumor Institute, Northwestern University Feinberg School of 
      Medicine, Chicago, IL.
FAU - Goldman, Stewart
AU  - Goldman S
AD  - Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, 
      Northwestern University Feinberg School of Medicine, Chicago, IL.
AD  - Division of Hematology and Oncology, Ann & Robert H. Lurie Children's Hospital of 
      Chicago, Chicago, IL.
FAU - Platanias, Leonidas C
AU  - Platanias LC
AD  - Robert H. Lurie Comprehensive Cancer Center and Division of Hematology-Oncology, 
      Northwestern University Feinberg School of Medicine, Chicago, IL.
AD  - Department of Medicine, Jesse Brown VA Medical Center, Chicago, IL.
LA  - eng
GR  - R01 CA121192/CA/NCI NIH HHS/United States
GR  - R01 CA077816/CA/NCI NIH HHS/United States
GR  - I01 CX000916/CX/CSRD VA/United States
GR  - R01 NS095634/NS/NINDS NIH HHS/United States
GR  - T32 CA009560/CA/NCI NIH HHS/United States
GR  - R01 CA155566/CA/NCI NIH HHS/United States
GR  - CA77816/CA/NCI NIH HHS/United States
GR  - P30 CA060553/CA/NCI NIH HHS/United States
GR  - R01 CA159811/CA/NCI NIH HHS/United States
GR  - R01 NS093843/NS/NINDS NIH HHS/United States
GR  - T32 CA070085/CA/NCI NIH HHS/United States
GR  - T32 GM008152/GM/NIGMS NIH HHS/United States
GR  - CA155566/CA/NCI NIH HHS/United States
GR  - R01 CA158911-S3/CA/NCI NIH HHS/United States
GR  - CA060553/CA/NCI NIH HHS/United States
GR  - T32 CA09560/CA/NCI NIH HHS/United States
GR  - R01 CA158911/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20160101
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Biological Assay/methods
MH  - Brain Neoplasms/diagnosis/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Glioblastoma/diagnosis/*pathology
MH  - Humans
MH  - Neoplastic Stem Cells/pathology
MH  - Spheroids, Cellular/pathology
MH  - Staining and Labeling/*methods
PMC - PMC4772731
MID - NIHMS761096
OTO - NOTNLM
OT  - acridine orange
OT  - cancer stem cell
OT  - glioblastoma
OT  - microscopy
OT  - neurospheres
OT  - tumor spheroids
EDAT- 2016/01/14 06:00
MHDA- 2016/10/07 06:00
PMCR- 2016/03/01
CRDT- 2016/01/14 06:00
PHST- 2015/05/11 00:00 [received]
PHST- 2015/09/23 00:00 [accepted]
PHST- 2016/01/14 06:00 [entrez]
PHST- 2016/01/14 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - 000114372 [pii]
AID - 10.2144/000114372 [doi]
PST - epublish
SO  - Biotechniques. 2016 Jan 1;60(1):43-6. doi: 10.2144/000114372. eCollection 2016 
      Jan.