PMID- 2675782
OWN - NLM
STAT- MEDLINE
DCOM- 19891020
LR  - 20190503
IS  - 0003-4967 (Print)
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 48
IP  - 8
DP  - 1989 Aug
TI  - Immunolocalization of matrix metalloproteinase 3 (stromelysin) in rheumatoid 
      synovioblasts (B cells): correlation with rheumatoid arthritis.
PG  - 645-53
AB  - Metalloproteinases produced by connective tissue cells may play a key part in the 
      destruction of joints in rheumatoid arthritis. Matrix metalloproteinase 3 (MMP-3; 
      stromelysin) capable of degrading cartilage proteoglycans and type IX collagen 
      and of activating procollagenase was immunolocalised in hyperplastic synovial 
      lining cells in rheumatoid synovium, but not in the cells of normal synovium. 
      Cells responsible for synthesis of MMP-3 have the phenotype of synovioblasts (B 
      cells) by immunoelectron microscopy, but not of phagocytic synovial macrophages 
      (A cells). Cultured monolayer of rheumatoid synovial cells synthesises MMP-3 only 
      under treatment with macrophage conditioned medium. Immunolocalisation of MMP-3 
      in rheumatoid synovium and cultured synovial cells was possible when the 
      specimens were treated with a monovalent ionophore, monensin. These results 
      suggest that MMP-3 is synthesised and secreted continuously without storage from 
      hyperplastic synovioblasts stimulated by factor(s) derived from activated 
      macrophages present in the synovium.
FAU - Okada, Y
AU  - Okada Y
AD  - Department of Pathology, School of Medicine, Kanazawa University, Ishikawa, 
      Japan.
FAU - Takeuchi, N
AU  - Takeuchi N
FAU - Tomita, K
AU  - Tomita K
FAU - Nakanishi, I
AU  - Nakanishi I
FAU - Nagase, H
AU  - Nagase H
LA  - eng
GR  - AR 39189/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Arthritis, Rheumatoid/*enzymology/immunology
MH  - B-Lymphocytes/*enzymology/immunology
MH  - Cells, Cultured
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Matrix Metalloproteinase 3
MH  - Metalloendopeptidases/*analysis/biosynthesis/immunology
MH  - Microscopy, Electron
MH  - Synovial Membrane/*enzymology/immunology
PMC - PMC1003840
EDAT- 1989/08/01 00:00
MHDA- 1989/08/01 00:01
PMCR- 1992/08/01
CRDT- 1989/08/01 00:00
PHST- 1989/08/01 00:00 [pubmed]
PHST- 1989/08/01 00:01 [medline]
PHST- 1989/08/01 00:00 [entrez]
PHST- 1992/08/01 00:00 [pmc-release]
AID - 10.1136/ard.48.8.645 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 1989 Aug;48(8):645-53. doi: 10.1136/ard.48.8.645.