PMID- 26758268
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20181202
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 41
IP  - 6
DP  - 2016 Jun
TI  - Heat shock protein 60 affects behavioral improvement in a rat model of 
      Parkinson's disease grafted with human umbilical cord mesenchymal stem 
      cell-derived dopaminergic-like neurons.
PG  - 1238-49
LID - 10.1007/s11064-015-1816-6 [doi]
AB  - Parkinson's disease (PD) is a neurodegenerative disorder that is caused by a loss 
      of dopaminergic (DAergic) neurons in mesencephalic substantia nigra (SN). Human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) are capable of self-renewal and 
      differentiation into multiple cell lineages, including DAergic neurons. Thus, 
      hUC-MSCs could be a promising alternative to compensate for the loss of DAergic 
      neurons in PD. In the current study, hUC-MSCs and hUC-MSCs-derived DAergic-like 
      neurons were transplanted into the striatum and SN of a rat model of PD that is 
      induced by 6-hydroxydopamine (6-OHDA). We evaluated their therapeutic effects on 
      improving rotation behavior in the rat and on modulating the level of heat shock 
      protein 60 (Hsp60) expression in the brain. After transplantation, an 
      amelioration of rotation behavior was observed in rats that underwent cell 
      grafting, and hUC-MSCs-derived DAergic-like neurons were superior to hUC-MSCs at 
      inducing behavioral improvement. Western blot and immunohistochemistry analysis 
      indicated significantly elevated levels of Hsp60 in cell-grafted rats compared to 
      6-OHDA-lesioned (PD) rats. These results demonstrate that hUC-MSCs-based cell 
      transplantation is potential therapeutic treatment for PD, and hUC-MSCs-derived 
      DAergic-like neurons appear to be favorable candidates for cell replacement 
      therapy in PD. Finally, Hsp60 could be involved in a mechanism of behavioral 
      recovery.
FAU - Zhao, Can
AU  - Zhao C
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China.
FAU - Li, Hui
AU  - Li H
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China.
FAU - Zhao, Xian-Jing
AU  - Zhao XJ
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China.
FAU - Liu, Zheng-Xia
AU  - Liu ZX
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China.
FAU - Zhou, Ping
AU  - Zhou P
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China.
FAU - Feng, Mei-Jiang
AU  - Feng MJ
AD  - Department of Geriatrics, the Second Affiliated Hospital, Nanjing Medical 
      University, Nanjing, 210011, Jiangsu, People's Republic of China. 
      mjfeng416@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20160112
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Chaperonin 60)
RN  - 0 (Hspd1 protein, rat)
RN  - 0 (Mitochondrial Proteins)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Cells, Cultured
MH  - Chaperonin 60/*biosynthesis
MH  - Corpus Striatum/metabolism
MH  - Dopaminergic Neurons/*transplantation
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/metabolism
MH  - Mitochondrial Proteins/*biosynthesis
MH  - Parkinsonian Disorders/*metabolism/*therapy
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Umbilical Cord/cytology/*transplantation
OTO - NOTNLM
OT  - Cell replacement therapy
OT  - Dopaminergic-like neuron
OT  - Heat shock protein 60
OT  - Human umbilical cord mesenchymal stem cell
OT  - Parkinson's disease
EDAT- 2016/01/14 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/01/14 06:00
PHST- 2015/05/11 00:00 [received]
PHST- 2015/12/23 00:00 [accepted]
PHST- 2015/11/19 00:00 [revised]
PHST- 2016/01/14 06:00 [entrez]
PHST- 2016/01/14 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - 10.1007/s11064-015-1816-6 [pii]
AID - 10.1007/s11064-015-1816-6 [doi]
PST - ppublish
SO  - Neurochem Res. 2016 Jun;41(6):1238-49. doi: 10.1007/s11064-015-1816-6. Epub 2016 
      Jan 12.