PMID- 26760907
OWN - NLM
STAT- MEDLINE
DCOM- 20161109
LR  - 20161230
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 259
DP  - 2016 Feb
TI  - Development of an analytical method for simultaneous detection of psychotropic 
      phenylalkylamines in hair by LC-MS/MS with a multi-mode reversed-phase column 
      using pH gradient elution.
PG  - 69-76
LID - S0379-0738(15)00506-X [pii]
LID - 10.1016/j.forsciint.2015.12.016 [doi]
AB  - Phenylalkylamine derivatives, such as methamphetamine (MA), 
      3,4-methylenedioxymethamphetamine (MDMA), phentermine, fenfluramine, 
      phendimetrazine, amfepramone, and ketamine, are widely abused recreational or 
      anorectic drugs in Korea, and their abuse has become a serious social problem. 
      Hair is a useful specimen to prove chronic use and liquid chromatography-tandem 
      mass spectrometry (LC-MS/MS) has recently become a more popular tool for hair 
      analysis due to sensitivity and simplicity in sample preparation. In order to 
      overcome limitations of standard reversed-phase column to separate low molecular 
      weight amines, we adopted a multi-mode reversed-phase column, Scherzo SS-C18, 
      which was composed of strong ionic ligands and C18 ligands, and used pH gradient 
      elution to separate seven psychotropic phenylalkylamines and their metabolites. 
      The essential validation parameters including selectivity, LOD, LLOQ, linearity, 
      intra- and inter-assay precision and accuracy, recovery, and the matrix effect 
      were satisfactory. The LODs ranged from 0.1ng/5mg hair (diethylnorephedrine, 
      fenfluramine, ketamine, and MA) to 0.5ng/5mg hair (amfepramone, MDA, 
      phendimetrazine, and phentermine). The LLOQs were 1ng/5mg hair for all analytes. 
      The developed method was successfully applied to determination of 
      phenylalkylamines in authentic hair samples analyzed previously by a routine gas 
      chromatography/mass spectrometry (GC-MS) method. A good correlation was observed 
      between the two methods, with a slope near one.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Choi, Hyeyoung
AU  - Choi H
AD  - Daejeon Institute, National Forensic Service , 1524 Yuseongdaero, Yuseong-gu, 
      Daejeon 34054, Republic of Korea; College of Pharmacy, Chungbuk National 
      University, 1 Chungdaero, Seowon-gu, Cheongju, Chungbuk 28356, Republic of Korea. 
      Electronic address: hychoi22@korea.kr.
FAU - Kim, Suncheun
AU  - Kim S
AD  - Drug & Forensic Toxicology Division, National Forensic Service, 10 Ipchunro, 
      Wonjusi, Gangwondo 26460, Republic of Korea. Electronic address: nisi@korea.kr.
FAU - Ahn, Suyoun
AU  - Ahn S
AD  - Daejeon Institute, National Forensic Service , 1524 Yuseongdaero, Yuseong-gu, 
      Daejeon 34054, Republic of Korea. Electronic address: aphroditesu@korea.kr.
FAU - Chang, Hyejin
AU  - Chang H
AD  - Daejeon Institute, National Forensic Service , 1524 Yuseongdaero, Yuseong-gu, 
      Daejeon 34054, Republic of Korea. Electronic address: changhj@korea.kr.
FAU - Lee, Sangki
AU  - Lee S
AD  - Daejeon Institute, National Forensic Service , 1524 Yuseongdaero, Yuseong-gu, 
      Daejeon 34054, Republic of Korea. Electronic address: skleedoc@korea.kr.
FAU - Lee, Yongmoon
AU  - Lee Y
AD  - College of Pharmacy, Chungbuk National University, 1 Chungdaero, Seowon-gu, 
      Cheongju, Chungbuk 28356, Republic of Korea. Electronic address: 
      ymleefn@cbnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151223
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Phenethylamines)
RN  - 0 (Psychotropic Drugs)
SB  - IM
MH  - Adult
MH  - Chromatography, Liquid/*methods
MH  - Chromatography, Reverse-Phase/*methods
MH  - Female
MH  - Hair/*chemistry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Male
MH  - Middle Aged
MH  - Phenethylamines/*analysis
MH  - Psychotropic Drugs/*analysis
MH  - Tandem Mass Spectrometry/*methods
MH  - Young Adult
OTO - NOTNLM
OT  - LC-MS/MS
OT  - Multi-mode reversed phase column
OT  - Psychotropic phenylalkylamines
OT  - pH gradient
EDAT- 2016/01/14 06:00
MHDA- 2016/11/10 06:00
CRDT- 2016/01/14 06:00
PHST- 2015/09/08 00:00 [received]
PHST- 2015/11/23 00:00 [revised]
PHST- 2015/12/11 00:00 [accepted]
PHST- 2016/01/14 06:00 [entrez]
PHST- 2016/01/14 06:00 [pubmed]
PHST- 2016/11/10 06:00 [medline]
AID - S0379-0738(15)00506-X [pii]
AID - 10.1016/j.forsciint.2015.12.016 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2016 Feb;259:69-76. doi: 10.1016/j.forsciint.2015.12.016. Epub 
      2015 Dec 23.