PMID- 26761396
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20170602
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 489
DP  - 2016 Feb
TI  - Effects of modification of the HIV-1 Env cytoplasmic tail on immunogenicity of 
      VLP vaccines.
PG  - 141-50
LID - S0042-6822(15)00406-7 [pii]
LID - 10.1016/j.virol.2015.09.015 [doi]
AB  - We investigated the effects on assembly and antigenic properties of specific 
      modifications of the transmembrane spanning (TMS) and cytoplasmic tail (CT) 
      domains of HIV-1 Env from a transmitted/founder (T/F) ZM53 Env glycoprotein. A 
      construct containing a short version of the TMS domain derived from the mouse 
      mammary tumor virus (MMTV) Env with or without a GCN4 trimerization sequence in 
      the CT exhibited the highest levels of incorporation into VLPs and induced the 
      highest titers of anti-Env IgG immune responses in a VLP context. Sera from 
      guinea pigs immunized by VLPs with high Env content, and containing the CT 
      trimerization sequence, had increased neutralization activity and antibody 
      avidity. A cross-clade prime-boost regimen with clade B SF162 or clade C ZM53 Env 
      DNA priming and boosting with VLPs containing modified ZM53 Env further enhanced 
      these immune responses. The modified VLPs demonstrate improved potential as HIV-1 
      vaccine antigens.
CI  - Copyright (c) 2016. Published by Elsevier Inc.
FAU - Vzorov, Andrei N
AU  - Vzorov AN
AD  - Department of Microbiology and Immunology and Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA 30322, USA. Electronic address: 
      avzorov@emory.edu.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Microbiology and Immunology and Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA 30322, USA.
FAU - Chen, Jianjun
AU  - Chen J
AD  - Department of Microbiology and Immunology and Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA 30322, USA.
FAU - Wang, Bao-Zhong
AU  - Wang BZ
AD  - Department of Microbiology and Immunology and Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA 30322, USA.
FAU - Compans, Richard W
AU  - Compans RW
AD  - Department of Microbiology and Immunology and Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA 30322, USA.
LA  - eng
GR  - AI090480/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160104
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (AIDS Vaccines)
RN  - 0 (HIV Antibodies)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - AIDS Vaccines/administration & dosage/chemistry/genetics/immunology
MH  - Amino Acid Motifs
MH  - Animals
MH  - Female
MH  - Guinea Pigs
MH  - HIV Antibodies/immunology
MH  - HIV Infections/immunology/*prevention & control/virology
MH  - HIV-1/chemistry/genetics/*immunology
MH  - Humans
MH  - Immunization
MH  - Mice
MH  - Neutralization Tests
MH  - env Gene Products, Human Immunodeficiency Virus/administration & 
      dosage/*chemistry/genetics/*immunology
OTO - NOTNLM
OT  - Antibody avidity
OT  - Cross-clade immunization
OT  - Cytoplasmic tail
OT  - Env glycoprotein
OT  - HIV-1
OT  - Immunogenicity
OT  - Neutralizing antibodies
OT  - Protein modification
OT  - Trimer stability
OT  - VLP vaccine
EDAT- 2016/01/14 06:00
MHDA- 2016/07/07 06:00
CRDT- 2016/01/14 06:00
PHST- 2015/07/09 00:00 [received]
PHST- 2015/09/23 00:00 [revised]
PHST- 2015/09/24 00:00 [accepted]
PHST- 2016/01/14 06:00 [entrez]
PHST- 2016/01/14 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
AID - S0042-6822(15)00406-7 [pii]
AID - 10.1016/j.virol.2015.09.015 [doi]
PST - ppublish
SO  - Virology. 2016 Feb;489:141-50. doi: 10.1016/j.virol.2015.09.015. Epub 2016 Jan 4.