PMID- 26761554
OWN - NLM
STAT- MEDLINE
DCOM- 20160805
LR  - 20181113
IS  - 2317-6385 (Electronic)
IS  - 1679-4508 (Print)
IS  - 1679-4508 (Linking)
VI  - 13
IP  - 4
DP  - 2015 Oct-Dec
TI  - Sensitization by subcutaneous route is superior to intraperitoneal route in 
      induction of asthma by house dust mite in a murine mode.
PG  - 560-6
LID - S1679-45082015000400560 [pii]
LID - 10.1590/S1679-45082015AO3389 [doi]
AB  - OBJECTIVE: To develop a new experimental model of chronic allergic pulmonary 
      disease induced by house dust mite, with marked production of specific 
      immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and 
      remodeling, comparing two different routes of sensitization. METHODS: The 
      protocol lasted 30 days. BALB/c mice were divided into six groups and were 
      sensitized subcutaneously or intraperitoneally with saline (negative control), 
      Dermatophagoides pteronyssinus (Der p) 50 or 500 mcg in three injections. 
      Subsequently they underwent intranasal challenge with Der p or saline for 7 days 
      and were sacrificed 24 hours after the last challenge. We evaluated the titration 
      of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and 
      airway remodeling. RESULTS: Both animals sensitized intraperitoneally and 
      subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia 
      increased only in mice receiving lower doses of Der p. However, only the group 
      sensitized with Der p 50 mcg through subcutaneously route showed significant 
      airway remodeling. CONCLUSION: In this murine model of asthma, both pathways of 
      sensitization led to the production of specific IgE and eosinophilia in the 
      airways. However, only the subcutaneously route was able to induce remodeling. 
      Furthermore, lower doses of Der p used in sensitization were better than higher 
      ones, suggesting immune tolerance. Further studies are required to evaluate the 
      efficacy of this model in the development of bronchial hyperresponsiveness, but 
      it can already be replicated in experiments to create new therapeutic drugs or 
      immunotherapeutic strategies.
FAU - Aun, Marcelo Vivolo
AU  - Aun MV
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Saraiva-Romanholo, Beatriz Mangueira
AU  - Saraiva-Romanholo BM
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Almeida, Francine Maria de
AU  - Almeida FM
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Bruggemann, Thayse Regina
AU  - Bruggemann TR
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Kalil, Jorge
AU  - Kalil J
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Martins, Milton de Arruda
AU  - Martins Mde A
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Arantes-Costa, Fernanda Magalhaes
AU  - Arantes-Costa FM
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Giavina-Bianchi, Pedro
AU  - Giavina-Bianchi P
AD  - Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
LA  - eng
LA  - por
PT  - Journal Article
PL  - Brazil
TA  - Einstein (Sao Paulo)
JT  - Einstein (Sao Paulo, Brazil)
JID - 101281800
RN  - 0 (Allergens)
RN  - 0 (Fibrillar Collagens)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Administration, Intranasal
MH  - Allergens/*administration & dosage
MH  - Animals
MH  - Asthma/*immunology/physiopathology
MH  - Bronchial Provocation Tests
MH  - *Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eosinophils/metabolism
MH  - Fibrillar Collagens/metabolism
MH  - Immunization/*methods
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Injections, Intraperitoneal
MH  - Injections, Subcutaneous
MH  - Leukocyte Count
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Passive Cutaneous Anaphylaxis/immunology
MH  - Pulmonary Eosinophilia/parasitology
MH  - *Pyroglyphidae
PMC - PMC4878631
COIS- Conflict of interest: none.
EDAT- 2016/01/14 06:00
MHDA- 2016/08/06 06:00
PMCR- 2015/10/01
CRDT- 2016/01/14 06:00
PHST- 2015/05/01 00:00 [received]
PHST- 2015/10/01 00:00 [accepted]
PHST- 2016/01/14 06:00 [entrez]
PHST- 2016/01/14 06:00 [pubmed]
PHST- 2016/08/06 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - S1679-45082015000400560 [pii]
AID - S1679-45082015AO3389 [pii]
AID - 10.1590/S1679-45082015AO3389 [doi]
PST - ppublish
SO  - Einstein (Sao Paulo). 2015 Oct-Dec;13(4):560-6. doi: 
      10.1590/S1679-45082015AO3389.