PMID- 26762031 OWN - NLM STAT- MEDLINE DCOM- 20160314 LR - 20160114 IS - 0001-6209 (Print) IS - 0001-6209 (Linking) VI - 55 IP - 9 DP - 2015 Sep 4 TI - [Relationship between bacterial community and its environmental in Zuohai Lake, China]. PG - 1177-89 AB - OBJECTIVE: To reveal the relationship between bacterial community and the environmental in Zuohai lake, Fuzhou City. METHODS: The abundance of total bacteria was studied using DAPI staining; the composition, distribution and the dynamics of bacterial community were examined using both the denaturing gradient gel electrophoresis (DGGE) and the 16S rRNA gene clone library analyses over a 2-year periods from October 2011 to August 2013. Moreover, multivariate statistical analysis was used to analyze the relationship between bacterial community and environmental factors. RESULTS: The total numbers of bacteria ranging from 4.91 x 10(6) cells/mL to 18.71 x 10(6) cells/mL, and the seasonal variation of bacterial abundance was obviously stronger than the spatial heterogeneity. DGGE and clone library analysis revealed that bacterial communities were mainly affected by temporal changes. Phylogenetic analysis showed that bacteria belonging to 11 phyla were identified. Members of 3-Proteobacteria and Cyanobacteria group were the predominated lineage, followed by a-Proteo bacteria, Actinobacteria and Bacteroidetes. Redundancy analysis (RDA) suggested that water temperature, pH, total nitrogen, total phosphorus and chlorophyll a content contributed significantly to the bacteria-environment relationship. CONCLUSION: There is rich bacterial diversity in Zuohai lake, the patterns of change in bacterial communities showed that the seasonal difference might have a significant impact on structuring the bacterial communities in the lake. FAU - Liu, Lanying AU - Liu L FAU - Lv, Xin AU - Lv X FAU - Li, Wei AU - Li W FAU - Chen, Lihua AU - Chen L FAU - Li, Yueren AU - Li Y FAU - Lin, Bijiao AU - Lin B LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Wei Sheng Wu Xue Bao JT - Wei sheng wu xue bao = Acta microbiologica Sinica JID - 21610860R RN - 0 (DNA, Bacterial) RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Bacteria/classification/genetics/*isolation & purification MH - *Biodiversity MH - China MH - DNA, Bacterial/genetics MH - Lakes/chemistry/*microbiology MH - Molecular Sequence Data MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics MH - Seasons EDAT- 2016/01/15 06:00 MHDA- 2016/03/15 06:00 CRDT- 2016/01/15 06:00 PHST- 2016/01/15 06:00 [entrez] PHST- 2016/01/15 06:00 [pubmed] PHST- 2016/03/15 06:00 [medline] PST - ppublish SO - Wei Sheng Wu Xue Bao. 2015 Sep 4;55(9):1177-89.