PMID- 26763434
OWN - NLM
STAT- MEDLINE
DCOM- 20160520
LR  - 20210103
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 136
IP  - 1
DP  - 2016 Jan
TI  - Early Tumor-Infiltrating Dendritic Cells Change their Characteristics Drastically 
      in Association with Murine Melanoma Progression.
PG  - 146-53
LID - S0022-202X(15)00009-3 [pii]
LID - 10.1038/JID.2015.359 [doi]
AB  - Dendritic cells (DCs) have a critical effect on the outcome of adaptive immune 
      responses against growing tumors. Tumor-infiltrating dendritic cells (TIDCs) play 
      diverse roles in the regulation of tumor regression or growth, but the 
      characteristics that distinguish those effects are obscure. In this study, we 
      investigated the frequency, phenotype, and function of TIDCs over time from early 
      stages of melanoma growth in mice. Flow cytometric analysis revealed that the 
      tumors were infiltrated by a significant population of CD11c(+) major 
      histocompatibility complex II(+) DCs, especially at an early stage of tumor 
      growth. The allogeneic stimulatory capacity of TIDCs increased with tumor growth, 
      whereas this capacity of DCs in lymph nodes decreased. TIDCs harvested at an 
      early stage of melanoma (early TIDCs) accelerated tumor growth, but those 
      harvested at a late stage (late TIDCs) delayed tumor progression when they were 
      coinjected with melanoma cells. Furthermore, coinjection of early TIDCs failed to 
      induce full immunocompetent maturation of CD8(+) T cells, with much lower 
      expression of IFN-gamma, granzyme B, and perforin within the tumor microenvironment. 
      In conclusion, TIDCs change their characteristics from an immunoinhibitory to an 
      immunostimulatory phenotype over time in association with tumor progression.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Nakahara, Takeshi
AU  - Nakahara T
AD  - Division of Skin Surface Sensing, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan; Department of Dermatology, Graduate School of Medical 
      Sciences, Kyushu University, Fukuoka, Japan. Electronic address: 
      nakahara@dermatol.med.kyushu-u.ac.jp.
FAU - Oba, Junna
AU  - Oba J
AD  - Department of Dermatology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Shimomura, Chie
AU  - Shimomura C
AD  - Department of Dermatology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Kido-Nakahara, Makiko
AU  - Kido-Nakahara M
AD  - Department of Dermatology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Furue, Masutaka
AU  - Furue M
AD  - Division of Skin Surface Sensing, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan; Department of Dermatology, Graduate School of Medical 
      Sciences, Kyushu University, Fukuoka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
SB  - IM
MH  - Animals
MH  - Cell Transformation, Neoplastic/*pathology
MH  - Dendritic Cells/cytology/*pathology
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Melanoma/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Random Allocation
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*pathology/physiopathology
MH  - *Tumor Burden
EDAT- 2016/01/15 06:00
MHDA- 2016/05/21 06:00
CRDT- 2016/01/15 06:00
PHST- 2015/03/12 00:00 [received]
PHST- 2015/08/13 00:00 [revised]
PHST- 2015/08/27 00:00 [accepted]
PHST- 2016/01/15 06:00 [entrez]
PHST- 2016/01/15 06:00 [pubmed]
PHST- 2016/05/21 06:00 [medline]
AID - S0022-202X(15)00009-3 [pii]
AID - 10.1038/JID.2015.359 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2016 Jan;136(1):146-53. doi: 10.1038/JID.2015.359.