PMID- 26768374
OWN - NLM
STAT- MEDLINE
DCOM- 20161228
LR  - 20220311
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 54
IP  - 4
DP  - 2016 May
TI  - Paracoccidioides brasiliensis interacts with dermal dendritic cells and 
      keratinocytes in human skin and oral mucosa lesions.
PG  - 370-6
LID - 10.1093/mmy/myv112 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic disease caused by the fungus 
      Paracoccidioides brasiliensis and Paracoccidioides lutzii. In PCM the skin and 
      oral mucosa are often affected. Dendritic cells and keratinocytes of the 
      integument play a role in innate and adaptive immune response against pathogens, 
      due to their function as antigen presenting cells. Aiming to verify the 
      interaction of P. brasiliensis with these cell populations, we studied 52 skin 
      and 47 oral mucosa samples taken from patients with proven diagnosis of PCM. The 
      biopsies were subjected to immunohistochemical and/or immunofluorescence staining 
      with anti-factor XIIIa (marker of dermal dendrocytes), anti-CD207 (marker of 
      mature Langerhans cells), anti-pan cytokeratins (AE1-AE3) and anti-P. 
      brasiliensis antibodies. Analyses with confocal laser microscopy were also 
      performed for better visualization of the interaction between keratinocytes and 
      the fungi. In sum, 42% of oral mucosa samples displayed yeast forms in Factor 
      XIIIa dermal dendrocytes cytoplasm. Langerhans cells in skin and oral mucosa 
      samples did not show yeast cells in their cytoplasm. In sum, 54% of skin and 60% 
      of mucosal samples displayed yeast cells in the cytoplasm of keratinocytes. The 
      parasitism of keratinocytes may represent a possible mechanism of evasion of the 
      fungus to local immune mechanisms. Factor XIIIa dendrocytes and keratinocytes may 
      be acting as antigen-presenting cells to fulfill the probably impaired function 
      of Langerhans cells in skin and oral mucosa of human PCM.
CI  - (c) The Author 2015. Published by Oxford University Press on behalf of The 
      International Society for Human and Animal Mycology. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Silva, Wellington Luiz Ferreira da
AU  - Silva WL
AD  - Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, 
      Brazil.
FAU - Pagliari, Carla
AU  - Pagliari C
AD  - Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, 
      Brazil Department of Pathology, University of Sao Paulo Medical School, Sao 
      Paulo, Brazil.
FAU - Duarte, Maria Irma Seixas
AU  - Duarte MI
AD  - Department of Pathology, University of Sao Paulo Medical School, Sao Paulo, 
      Brazil.
FAU - Sotto, Mirian N
AU  - Sotto MN
AD  - Department of Dermatology, University of Sao Paulo Medical School, Sao Paulo, 
      Brazil Department of Pathology, University of Sao Paulo Medical School, Sao 
      Paulo, Brazil mnsotto@usp.br.
LA  - eng
PT  - Journal Article
DEP - 20160114
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
SB  - IM
MH  - *Dendritic Cells/immunology/microbiology
MH  - Host-Pathogen Interactions/*immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - *Keratinocytes/immunology/microbiology
MH  - *Mouth Mucosa/cytology/immunology
MH  - Paracoccidioides/*immunology
MH  - *Skin/cytology/immunology
OTO - NOTNLM
OT  - Paracoccidioidomycosis
OT  - confocal laser microscopy
OT  - dendritic cells
OT  - immunohistochemistry
OT  - keratinocytes
OT  - skin and oral mucosa
EDAT- 2016/01/16 06:00
MHDA- 2016/12/29 06:00
CRDT- 2016/01/16 06:00
PHST- 2015/12/08 00:00 [accepted]
PHST- 2015/08/24 00:00 [received]
PHST- 2016/01/16 06:00 [entrez]
PHST- 2016/01/16 06:00 [pubmed]
PHST- 2016/12/29 06:00 [medline]
AID - myv112 [pii]
AID - 10.1093/mmy/myv112 [doi]
PST - ppublish
SO  - Med Mycol. 2016 May;54(4):370-6. doi: 10.1093/mmy/myv112. Epub 2016 Jan 14.