PMID- 26769838
OWN - NLM
STAT- MEDLINE
DCOM- 20161024
LR  - 20161230
IS  - 1899-1505 (Electronic)
IS  - 0867-5910 (Linking)
VI  - 66
IP  - 6
DP  - 2015 Dec
TI  - Combination of hyperhomocysteinemia and ischemic tolerance in experimental model 
      of global ischemia in rats.
PG  - 887-97
AB  - Epidemiological studies show positive relationship between mild-to-moderate 
      hyperhomocysteinemia (hHcy) and the risk of cerebrovascular diseases. The study 
      determines whether hyperhomocysteinemia (risk factor of brain ischemia) alone or 
      in combination with the ischemic preconditioning (IPC) affects the 
      ischemia-induced neurodegenerative changes and imbalance in MAPK/p-ERK1/2 and 
      MAPK/p-p38 expression in the rat brains. hHcy was induced by subcutaneous 
      administration of homocysteine (0.45 mumol/g body weight) twice a day at 8 h 
      intervals for 14 days. Rats were preconditioned by 5 min ischemia and 2 days 
      later, 15 min of global forebrain ischemia was induced by four vessel occlusion. 
      We observed that hHcy alone significantly increased neurodegeneration by 
      Fluoro-Jade C and TUNEL possitive cells in hippocampus as well as in cortex. We 
      found elevated level of MAPK/p-ERK and decreased level of MAPK/p-p38 after 
      pre-ischemic challenge by Western blot and fluorescent immunohistochemistry. In 
      conclusion, preconditioning even if combined with hHcy could preserve the 
      neuronal tissue from lethal ischemic effect. This study provides evidence for the 
      interplay and tight integration between ERK and p38 MAPKs signalling mechanisms 
      in response to the hHcy and also if in association with brain ischemia/IPC 
      challenge in the rat brain.
FAU - Kovalska, M
AU  - Kovalska M
AD  - Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University, Martin, Slovakia.
FAU - Kovalska, L
AU  - Kovalska L
AD  - Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, 
      Comenius University, Martin, Slovakia.
FAU - Tothova, B
AU  - Tothova B
AD  - Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University, Martin, Slovakia.
FAU - Mahmood, S
AU  - Mahmood S
AD  - Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University, Martin, Slovakia.
AD  - BioMed, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
FAU - Adamkov, M
AU  - Adamkov M
AD  - Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University, Martin, Slovakia.
FAU - Lehotsky, J
AU  - Lehotsky J
AD  - Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University, Martin, Slovakia. lehotsky@jfmed.uniba.sk.
AD  - BioMed, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - J Physiol Pharmacol
JT  - Journal of physiology and pharmacology : an official journal of the Polish 
      Physiological Society
JID - 9114501
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Brain/blood supply/metabolism
MH  - Brain Ischemia/*metabolism
MH  - Disease Models, Animal
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/*metabolism
MH  - *Ischemic Preconditioning
MH  - Male
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Rats, Wistar
EDAT- 2016/01/16 06:00
MHDA- 2016/10/25 06:00
CRDT- 2016/01/16 06:00
PHST- 2015/06/24 00:00 [received]
PHST- 2015/10/15 00:00 [accepted]
PHST- 2016/01/16 06:00 [entrez]
PHST- 2016/01/16 06:00 [pubmed]
PHST- 2016/10/25 06:00 [medline]
PST - ppublish
SO  - J Physiol Pharmacol. 2015 Dec;66(6):887-97.