PMID- 26774522
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1872-7913 (Electronic)
IS  - 0924-8579 (Linking)
VI  - 47
IP  - 2
DP  - 2016 Feb
TI  - Reinforcement of the bactericidal effect of ciprofloxacin on Pseudomonas 
      aeruginosa biofilm by hyperbaric oxygen treatment.
PG  - 163-7
LID - S0924-8579(15)00420-3 [pii]
LID - 10.1016/j.ijantimicag.2015.12.005 [doi]
AB  - Chronic Pseudomonas aeruginosa lung infection is the most severe complication in 
      cystic fibrosis patients. It is characterised by antibiotic-tolerant biofilms in 
      the endobronchial mucus with zones of oxygen (O2) depletion mainly due to 
      polymorphonuclear leucocyte activity. Whilst the exact mechanisms affecting 
      antibiotic effectiveness on biofilms remain unclear, accumulating evidence 
      suggests that the efficacy of several bactericidal antibiotics such as 
      ciprofloxacin is enhanced by stimulation of the aerobic respiration of pathogens, 
      and that lack of O2 increases their tolerance. Reoxygenation of O2-depleted 
      biofilms may thus improve susceptibility to ciprofloxacin possibly by restoring 
      aerobic respiration. We tested such a strategy using reoxygenation of O2-depleted 
      P. aeruginosa strain PAO1 agarose-embedded biofilms by hyperbaric oxygen 
      treatment (HBOT) (100% O2, 2.8bar), enhancing the diffusive supply for aerobic 
      respiration during ciprofloxacin treatment. This proof-of-principle study 
      demonstrates that biofilm reoxygenation by HBOT can significantly enhance the 
      bactericidal activity of ciprofloxacin on P. aeruginosa. Combining ciprofloxacin 
      treatment with HBOT thus clearly has potential to improve the treatment of P. 
      aeruginosa biofilm infections.
CI  - Copyright (c) 2015 Elsevier B.V. and the International Society of Chemotherapy. All 
      rights reserved.
FAU - Kolpen, Mette
AU  - Kolpen M
AD  - Department of Clinical Microbiology, Rigshospitalet, Juliane Mariesvej 22, 2100 
      Copenhagen, Denmark; Department of International Health, Immunology and 
      Microbiology, UC-CARE, Faculty of Health Sciences, University of Copenhagen, 2200 
      Copenhagen, Denmark.
FAU - Mousavi, Nabi
AU  - Mousavi N
AD  - Department of Clinical Microbiology, Rigshospitalet, Juliane Mariesvej 22, 2100 
      Copenhagen, Denmark.
FAU - Sams, Thomas
AU  - Sams T
AD  - Biomedical Engineering, Department of Electrical Engineering, Technical 
      University of Denmark, 2800 Lyngby, Denmark.
FAU - Bjarnsholt, Thomas
AU  - Bjarnsholt T
AD  - Department of Clinical Microbiology, Rigshospitalet, Juliane Mariesvej 22, 2100 
      Copenhagen, Denmark; Department of International Health, Immunology and 
      Microbiology, UC-CARE, Faculty of Health Sciences, University of Copenhagen, 2200 
      Copenhagen, Denmark.
FAU - Ciofu, Oana
AU  - Ciofu O
AD  - Department of International Health, Immunology and Microbiology, UC-CARE, Faculty 
      of Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
FAU - Moser, Claus
AU  - Moser C
AD  - Department of Clinical Microbiology, Rigshospitalet, Juliane Mariesvej 22, 2100 
      Copenhagen, Denmark.
FAU - Kuhl, Michael
AU  - Kuhl M
AD  - Marine Biological Section, Department of Biology, University of Copenhagen, 3000 
      Helsingor, Denmark; Plant Functional Biology and Climate Change Cluster, 
      University of Technology Sydney, Australia.
FAU - Hoiby, Niels
AU  - Hoiby N
AD  - Department of Clinical Microbiology, Rigshospitalet, Juliane Mariesvej 22, 2100 
      Copenhagen, Denmark; Department of International Health, Immunology and 
      Microbiology, UC-CARE, Faculty of Health Sciences, University of Copenhagen, 2200 
      Copenhagen, Denmark.
FAU - Jensen, Peter Ostrup
AU  - Jensen PO
AD  - Department of Clinical Microbiology, Rigshospitalet, Juliane Mariesvej 22, 2100 
      Copenhagen, Denmark. Electronic address: peter.oestrup.jensen@regionh.dk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151229
PL  - Netherlands
TA  - Int J Antimicrob Agents
JT  - International journal of antimicrobial agents
JID - 9111860
RN  - 0 (Anti-Bacterial Agents)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aerobiosis
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Biofilms/*drug effects
MH  - Ciprofloxacin/*pharmacology
MH  - *Hydrostatic Pressure
MH  - Microbial Viability/*drug effects
MH  - Oxygen/*pharmacology
MH  - Pseudomonas aeruginosa/*drug effects/physiology
OTO - NOTNLM
OT  - Biofilm
OT  - Ciprofloxacin
OT  - Cystic fibrosis
OT  - Hyperbaric oxygen treatment
OT  - Pseudomonas aeruginosa
EDAT- 2016/01/18 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/01/18 06:00
PHST- 2015/10/06 00:00 [received]
PHST- 2015/12/03 00:00 [revised]
PHST- 2015/12/09 00:00 [accepted]
PHST- 2016/01/18 06:00 [entrez]
PHST- 2016/01/18 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0924-8579(15)00420-3 [pii]
AID - 10.1016/j.ijantimicag.2015.12.005 [doi]
PST - ppublish
SO  - Int J Antimicrob Agents. 2016 Feb;47(2):163-7. doi: 
      10.1016/j.ijantimicag.2015.12.005. Epub 2015 Dec 29.