PMID- 26775747
OWN - NLM
STAT- MEDLINE
DCOM- 20170411
LR  - 20181202
IS  - 1532-6500 (Electronic)
IS  - 1058-2746 (Linking)
VI  - 25
IP  - 6
DP  - 2016 Jun
TI  - Association of synovial inflammation and inflammatory mediators with glenohumeral 
      rotator cuff pathology.
PG  - 989-97
LID - S1058-2746(15)00582-0 [pii]
LID - 10.1016/j.jse.2015.10.011 [doi]
AB  - HYPOTHESIS: We hypothesized that patients with full-thickness rotator cuff tears 
      would have greater synovial inflammation compared with those without rotator cuff 
      tear pathology, with gene expression relating to histologic findings. METHODS: 
      Synovial sampling was performed in 19 patients with full-thickness rotator cuff 
      tears (RTC group) and in 11 patients without rotator cuff pathology (control 
      group). Cryosections were stained and examined under light microscopy and 
      confocal fluorescent microscopy for anti-cluster CD45 (common leukocyte antigen), 
      anti-CD31 (endothelial), and anti-CD68 (macrophage) cell surface markers. A 
      grading system was used to quantitate synovitis under light microscopy, and 
      digital image analysis was used to quantify the immunofluorescence staining area. 
      Quantitative polymerase chain reaction was performed for validated inflammatory 
      markers. Data were analyzed with analysis of covariance, Mann-Whitney U, and 
      Spearman rank order testing, with significance set at alpha = .05. RESULTS: The 
      synovitis score was significantly increased in the RTC group compared with 
      controls. Immunofluorescence demonstrated significantly increased staining for 
      CD31, CD45, and CD68 in the RTC vs control group. CD45+/68- cells were found 
      perivascularly, with CD45+/68+ cells toward the joint lining edge of the 
      synovium. Levels of matrix metalloproteinase-3 (MMP-3) and interleukin-6 were 
      significantly increased in the RTC group, with a positive correlation between the 
      synovitis score and MMP-3 expression. CONCLUSIONS: Patients with full-thickness 
      rotator cuff tears have greater levels of synovial inflammation, angiogenesis, 
      and MMP-3 upregulation compared with controls. Gene expression of MMP-3 
      correlates with the degree of synovitis.
CI  - Published by Elsevier Inc.
FAU - Abrams, Geoffrey D
AU  - Abrams GD
AD  - Department of Orthopedic Surgery, Stanford University, Stanford, CA, USA; VA Palo 
      Alto Healthcare System, Palo Alto, CA, USA. Electronic address: 
      gabrams@stanford.edu.
FAU - Luria, Ayala
AU  - Luria A
AD  - VA Palo Alto Healthcare System, Palo Alto, CA, USA.
FAU - Carr, Rebecca A
AU  - Carr RA
AD  - VA Palo Alto Healthcare System, Palo Alto, CA, USA.
FAU - Rhodes, Christopher
AU  - Rhodes C
AD  - VA Palo Alto Healthcare System, Palo Alto, CA, USA.
FAU - Robinson, William H
AU  - Robinson WH
AD  - VA Palo Alto Healthcare System, Palo Alto, CA, USA; Division of 
      Immunology/Rheumatology, Stanford University, Stanford, CA, USA.
FAU - Sokolove, Jeremy
AU  - Sokolove J
AD  - VA Palo Alto Healthcare System, Palo Alto, CA, USA; Division of 
      Immunology/Rheumatology, Stanford University, Stanford, CA, USA.
LA  - eng
PT  - Journal Article
DEP - 20160114
PL  - United States
TA  - J Shoulder Elbow Surg
JT  - Journal of shoulder and elbow surgery
JID - 9206499
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Biomarkers)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/analysis/genetics
MH  - Antigens, Differentiation, Myelomonocytic/analysis/genetics
MH  - Biomarkers/analysis
MH  - Case-Control Studies
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Inflammation Mediators/analysis
MH  - Interleukin-6/metabolism
MH  - Leukocyte Common Antigens/analysis/genetics
MH  - Male
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Middle Aged
MH  - Platelet Endothelial Cell Adhesion Molecule-1/analysis/genetics
MH  - Rotator Cuff Injuries/*complications
MH  - Severity of Illness Index
MH  - Synovial Membrane/chemistry/pathology
MH  - Synovitis/etiology/*genetics/*metabolism/pathology
MH  - Up-Regulation
OTO - NOTNLM
OT  - MMP
OT  - Rotator cuff
OT  - cytokine
OT  - inflammation
OT  - synovitis
OT  - synovium
EDAT- 2016/01/19 06:00
MHDA- 2017/04/12 06:00
CRDT- 2016/01/19 06:00
PHST- 2015/07/29 00:00 [received]
PHST- 2015/10/06 00:00 [revised]
PHST- 2015/10/18 00:00 [accepted]
PHST- 2016/01/19 06:00 [entrez]
PHST- 2016/01/19 06:00 [pubmed]
PHST- 2017/04/12 06:00 [medline]
AID - S1058-2746(15)00582-0 [pii]
AID - 10.1016/j.jse.2015.10.011 [doi]
PST - ppublish
SO  - J Shoulder Elbow Surg. 2016 Jun;25(6):989-97. doi: 10.1016/j.jse.2015.10.011. 
      Epub 2016 Jan 14.