PMID- 26780079
OWN - NLM
STAT- MEDLINE
DCOM- 20161020
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 106
DP  - 2015 Dec 25
TI  - Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow 
      Focus Device.
PG  - e53516
LID - 10.3791/53516 [doi]
LID - 53516
AB  - Double emulsions are useful in a number of biological and industrial applications 
      in which it is important to have an aqueous carrier fluid. This paper presents a 
      polydimethylsiloxane (PDMS) microfluidic device capable of generating 
      water/oil/water double emulsions using a coaxial flow focusing geometry that can 
      be fabricated entirely using soft lithography. Similar to emulsion devices using 
      glass capillaries, double emulsions can be formed in channels with uniform 
      wettability and with dimensions much smaller than the channel sizes. Three 
      dimensional flow focusing geometry is achieved by casting a pair of PDMS slabs 
      using two layer soft lithography, then mating the slabs together in a clamshell 
      configuration. Complementary locking features molded into the PDMS slabs enable 
      the accurate registration of features on each of the slab surfaces. Device 
      testing demonstrates formation of double emulsions from 14 microm to 50 microm in 
      diameter while using large channels that are robust against fouling and clogging.
FAU - Cole, Russell H
AU  - Cole RH
AD  - Department of Pharmaceutical Chemistry, University of California, San Francisco.
FAU - Tran, Tuan M
AU  - Tran TM
AD  - Joint UCSF/UCB Bioengineering Graduate Group, University of California, San 
      Francisco.
FAU - Abate, Adam R
AU  - Abate AR
AD  - Department of Bioengineering and Therapeutic Sciences, University of California, 
      San Francisco; California Institute for Quantitative Biosciences, University of 
      California, San Francisco; adam.abate@ucsf.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Video-Audio Media
DEP - 20151225
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Emulsions)
RN  - 059QF0KO0R (Water)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Emulsions/chemistry
MH  - Equipment Design
MH  - Glass/chemistry
MH  - Lab-On-A-Chip Devices
MH  - Microfluidic Analytical Techniques/*instrumentation/methods
MH  - Water/chemistry
MH  - Wettability
PMC - PMC4780863
EDAT- 2016/01/19 06:00
MHDA- 2016/10/21 06:00
PMCR- 2017/12/25
CRDT- 2016/01/19 06:00
PHST- 2016/01/19 06:00 [entrez]
PHST- 2016/01/19 06:00 [pubmed]
PHST- 2016/10/21 06:00 [medline]
PHST- 2017/12/25 00:00 [pmc-release]
AID - 53516 [pii]
AID - 10.3791/53516 [doi]
PST - epublish
SO  - J Vis Exp. 2015 Dec 25;(106):e53516. doi: 10.3791/53516.