PMID- 26780689
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 122
DP  - 2016 Mar
TI  - A simple protocol for attenuating the auto-fluorescence of cyanobacteria for 
      optimized fluorescence in situ hybridization (FISH) imaging.
PG  - 16-9
LID - S0167-7012(16)30008-2 [pii]
LID - 10.1016/j.mimet.2016.01.006 [doi]
AB  - Cyanobacteria contain pigments, which generate auto-fluorescence that interferes 
      with fluorescence in situ hybridization (FISH) imaging of cyanobacteria. We 
      describe simple chemical treatments using CuSO4 or H2O2 that significantly reduce 
      the auto-fluorescence of Microcystis strains. These protocols were successfully 
      applied in FISH experiments using 16S rRNA specific probes and filamentous 
      cyanobacteria.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Zeller, Perrine
AU  - Zeller P
AD  - INSERM U1193, Physiopathogenese et Traitement des Maladies du Foie, Hopital Paul 
      Brousse, batiment Lavoisier, 14 avenue Paul Vaillant Couturier, 94807 Villejuif 
      Cedex, France.
FAU - Ploux, Olivier
AU  - Ploux O
AD  - Laboratoire des Energies de Demain, LIED, UMR 8236 CNRS, Universite Paris 
      Diderot-Paris 7, 75013 Paris, France; Chimie ParisTech, 75005 Paris, France.
FAU - Mejean, Annick
AU  - Mejean A
AD  - Laboratoire des Energies de Demain, LIED, UMR 8236 CNRS, Universite Paris 
      Diderot-Paris 7, 75013 Paris, France. Electronic address: 
      annick.mejean@univ-paris-diderot.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160115
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - LRX7AJ16DT (Copper Sulfate)
SB  - IM
MH  - Bacteriological Techniques/*methods
MH  - Base Sequence
MH  - Copper Sulfate/chemistry
MH  - Cyanobacteria/*chemistry/genetics/isolation & purification
MH  - Fluorescence
MH  - Hydrogen Peroxide/pharmacology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microcystis/drug effects/genetics
MH  - Microscopy, Fluorescence
MH  - Oligonucleotide Probes/genetics
MH  - Optical Imaging/*methods
MH  - Permeability
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Auto-fluorescence
OT  - CuSO(4)
OT  - Cyanobacteria
OT  - H(2)O(2)
OT  - Microcystis
OT  - fluorescence in situ hybridization
EDAT- 2016/01/19 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/01/19 06:00
PHST- 2015/12/21 00:00 [received]
PHST- 2016/01/14 00:00 [revised]
PHST- 2016/01/14 00:00 [accepted]
PHST- 2016/01/19 06:00 [entrez]
PHST- 2016/01/19 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0167-7012(16)30008-2 [pii]
AID - 10.1016/j.mimet.2016.01.006 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2016 Mar;122:16-9. doi: 10.1016/j.mimet.2016.01.006. Epub 
      2016 Jan 15.