PMID- 26781360
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 166
DP  - 2016 Mar
TI  - Luteal P4 synthesis in early pregnant gilts after induction of estrus with 
      PMSG/hCG.
PG  - 28-35
LID - S0378-4320(16)30001-X [pii]
LID - 10.1016/j.anireprosci.2016.01.001 [doi]
AB  - The present study was designed to examine whether an estrus induction with 
      gonadotropins could affect luteal P4 synthesis in early pregnant gilts. Sixteen 
      prepubertal gilts received 750IU of PMSG and 500IU of hCG 72h later. Prepubertal 
      gilts in the control group (n=17) were observed daily for estrus behavior. All 
      gilts were inseminated in their first estrus. Corpora lutea (CLs) were collected 
      on days 10, 12 and 15 of pregnancy and analyzed for (1) the mRNA and protein 
      expression of steroidogenic acute regulatory protein (StAR), cytochrome P450 
      family 11 subfamily A polypeptide 1 (CYP11A1), and 3beta-hydroxysteroid 
      dehydrogenase (3betaHSD); (2) the tissue concentration of P4; and (3) the mRNA 
      expression of luteinizing hormone receptor (LHR) and estrogen receptors (ESR1 and 
      ESR2). Additionally, P4 concentration was analyzed in blood serum of all animals. 
      PMSG/hCG injections to induce estrus decreased mRNA expression of StAR, CYP11A1 
      and 3betaHSD on day 10 and CYP11A1 on day 12 of pregnancy compared with the control 
      group, while CYP11A1 and 3betaHSD proteins were down-regulated on day 10 in the 
      hormonally-treated gilts. Concentrations of P4 in luteal tissue and blood serum 
      were also lower in animals after gonadotropin-induced estrus. In contrast, LHR 
      and ESR1 mRNA expression was greater in PMSG/hCG-treated than control gilts on 
      day 15 of gestation. In conclusion, induction of estrus with a PMSG/hCG protocol 
      in prepubertal gilts impaired expression of the luteal P4 synthesis system. Low 
      P4 content may, in turn, induce local mechanisms involving LHR and ESR1 
      expression to support CL function.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Blitek, Agnieszka
AU  - Blitek A
AD  - Institute of Animal Reproduction and Food Research of the Polish Academy of 
      Sciences, Tuwima 10, 10-748 Olsztyn, Poland. Electronic address: 
      a.blitek@pan.olsztyn.pl.
FAU - Szymanska, Magdalena
AU  - Szymanska M
AD  - Institute of Animal Reproduction and Food Research of the Polish Academy of 
      Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
FAU - Pieczywek, Marta
AU  - Pieczywek M
AD  - Institute of Animal Reproduction and Food Research of the Polish Academy of 
      Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
FAU - Morawska-Pucinska, Ewa
AU  - Morawska-Pucinska E
AD  - Institute of Animal Reproduction and Food Research of the Polish Academy of 
      Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20160106
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Drug Combinations)
RN  - 0 (Gonadotropins, Equine)
RN  - 0 (PMSG-HCG)
RN  - 0 (Phosphoproteins)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/genetics/metabolism
MH  - Animals
MH  - Cholesterol Side-Chain Cleavage Enzyme/genetics/metabolism
MH  - Chorionic Gonadotropin/*therapeutic use
MH  - Corpus Luteum/*metabolism
MH  - Drug Combinations
MH  - *Estrus Synchronization/physiology
MH  - Female
MH  - Gonadotropins, Equine/*therapeutic use
MH  - Luteal Phase/genetics/metabolism
MH  - Ovulation Induction/*methods/veterinary
MH  - Phosphoproteins/genetics/metabolism
MH  - Pregnancy
MH  - Progesterone/*biosynthesis/metabolism
MH  - *Swine/genetics/metabolism
OTO - NOTNLM
OT  - Corpus luteum
OT  - Estrus induction
OT  - Pig
OT  - Pregnancy
OT  - Progesterone synthesis
EDAT- 2016/01/20 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/01/20 06:00
PHST- 2015/03/26 00:00 [received]
PHST- 2015/12/11 00:00 [revised]
PHST- 2016/01/04 00:00 [accepted]
PHST- 2016/01/20 06:00 [entrez]
PHST- 2016/01/20 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0378-4320(16)30001-X [pii]
AID - 10.1016/j.anireprosci.2016.01.001 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2016 Mar;166:28-35. doi: 10.1016/j.anireprosci.2016.01.001. Epub 
      2016 Jan 6.