PMID- 26781410
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20231111
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 18
IP  - 1
DP  - 2016 Jan
TI  - [Detection of copy number variations in pediatric ETV6/RUNX1-positive acute 
      lymphoblastic leukemia with multiplex ligation-dependent probe amplification].
PG  - 34-8
AB  - OBJECTIVE: To investigate the application of multiplex ligation-dependent probe 
      amplification (MLPA) in the detection of copy number variations (CNVs) in 
      pediatric ETV6/RUNX1-positive acute lymphoblastic leukemia (ALL), to compare this 
      method with conventional karyotype analysis and fluorescence in situ 
      hybridization (FISH), and to evaluate the value of MLPA. METHODS: The clinical 
      data of 95 children with ETV6/RUNX1-positive ALL who were treated from January 
      2006 to November 2012 were analyzed retrospectively, including clinical features, 
      results of karyotype analysis, and results of FISH. CNVs were detected with MLPA. 
      RESULTS: CNVs were detected in 73 (77%), and the median number of CNVs was 1 
      (range 0-6). The CNVs of EBF1, CDKN2A/2B, PAX5, ETV6, RB1, and BTG1 were detected 
      in more than 10% of all the patients. The changes in the chromosome segments 
      carrying the genes with CNVs detected by MLPA were not detected by conventional 
      karyotype analysis. The coincidence rate between the CNVs in ETV6 gene detected 
      by FISH and those detected by MLPA was 66%. CONCLUSIONS: MLPA is an efficient and 
      convenient method to detect CNVs in children with ETV6/RUNX1-positive ALL.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Pediatrics, Institute of Hematology and Blood Diseases Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 
      300020, China. xfzhu1981@126.com.
FAU - Liu, Xiao-Ming
AU  - Liu XM
FAU - Guo, Ye
AU  - Guo Y
FAU - Yang, Wen-Yu
AU  - Yang WY
FAU - Zhang, Jia-Yuan
AU  - Zhang JY
FAU - Liu, Fang
AU  - Liu F
FAU - Liu, Tian-Feng
AU  - Liu TF
FAU - Wang, Shu-Chun
AU  - Wang SC
FAU - Chen, Xiao-Juan
AU  - Chen XJ
FAU - Ruan, Min
AU  - Ruan M
FAU - Qi, Ben-Quan
AU  - Qi BQ
FAU - Chang, Li-Xian
AU  - Chang LX
FAU - Zou, Yao
AU  - Zou Y
FAU - Chen, Yu-Mei
AU  - Chen YM
FAU - Zhu, Xiao-Fan
AU  - Zhu XF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Core Binding Factor Alpha 2 Subunit/*analysis
MH  - *DNA Copy Number Variations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Oncogene Proteins, Fusion/*analysis
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
PMC - PMC7390102
EDAT- 2016/01/20 06:00
MHDA- 2016/03/15 06:00
PMCR- 2016/01/15
CRDT- 2016/01/20 06:00
PHST- 2016/01/20 06:00 [entrez]
PHST- 2016/01/20 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
PHST- 2016/01/15 00:00 [pmc-release]
AID - 10.7499/j.issn.1008-8830.2016.01.008 [pii]
AID - zgddekzz-18-1-34 [pii]
AID - 10.7499/j.issn.1008-8830.2016.01.008 [doi]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2016 Jan;18(1):34-8. doi: 
      10.7499/j.issn.1008-8830.2016.01.008.