PMID- 26781704
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20181113
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 78
IP  - 4
DP  - 2016 May 3
TI  - Analysis of genetic variation of porcine reproductive and respiratory syndrome 
      virus (PRRSV) isolates in Central China.
PG  - 641-8
LID - 10.1292/jvms.15-0570 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is an epidemic 
      etiology in pigs of all ages causing reproductive failure and respiratory 
      manifestation. PRRSV has been circulating in Chinese pig farms for almost 20 
      years. The aim of the present study was to fully understand the extent of the 
      genetic diversity and molecular characteristics of PRRSVs in Central China. A 
      strain of PRRSV isolated from a recent outbreak farm in Hunan province in Central 
      China, designated HUN-2014, was sequenced and analyzed with 39 other PRRSVs from 
      1998 to 2014 in Central China. Comparative results of genomic sequences revealed 
      that all 40 PRRSVs belonged to the North American genotype (NA genotype) and 
      shared 88.8-99.0% homology. Phylogenetic analysis showed three subgenotypes, 
      namely conventional PRRSV (C-PRRSV), specially mutant PRRSV (S-PRRSV) and highly 
      pathogenic PRRSV (HP-PRRSV), in all 40 PRRSVs. Moreover, comparative analysis of 
      amino acid (AA) sequences of NSP2, GP3, GP5 and ORF5a revealed the main evolution 
      trend of PRRSVs in Central China from 1998 to 2014, which was from C-PRRSV to 
      HP-PRRSV, accompanied by different evolving directions to S-PRRSV. In conclusion, 
      both the major evolutionary trend and special features of genetic variation 
      should be emphasized as theoretical basis for development of new vaccines and 
      control strategies for PRRS.
FAU - Liu, Can
AU  - Liu C
AD  - Department of Inspection Technology Research, China Institute of Veterinary Drug 
      Control, Beijing 100081, China.
FAU - Ning, Yibao
AU  - Ning Y
FAU - Xu, Binrui
AU  - Xu B
FAU - Gong, Wenzhi
AU  - Gong W
FAU - Zhang, Dongdong
AU  - Zhang D
LA  - eng
PT  - Journal Article
DEP - 20160115
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China
MH  - DNA, Viral
MH  - Endemic Diseases
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Molecular Typing
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/isolation & purification
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - Viral Proteins/chemistry/genetics
PMC - PMC4873856
EDAT- 2016/01/20 06:00
MHDA- 2017/06/06 06:00
PMCR- 2016/04/01
CRDT- 2016/01/20 06:00
PHST- 2016/01/20 06:00 [entrez]
PHST- 2016/01/20 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - 15-0570 [pii]
AID - 10.1292/jvms.15-0570 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2016 May 3;78(4):641-8. doi: 10.1292/jvms.15-0570. Epub 2016 Jan 
      15.