PMID- 26782475
OWN - NLM
STAT- MEDLINE
DCOM- 20170111
LR  - 20170112
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Dec 28
TI  - Effect of Ginkgo biloba extract on matrix metalloproteinase-3 expression in a rat 
      model of chondrocyte injury.
PG  - 18280-6
LID - 10.4238/2015.December.23.15 [doi]
AB  - A rat model with cartilage chondrocyte injury was established using 
      interleukin-1beta (IL-1beta) to investigate the effect of Ginkgo biloba extract (EGb) 
      on matrix metalloproteinase-3 (MMP-3) expression. Rat chondrocytes were extracted 
      and randomly divided into six groups: control group, IL-1beta (model) group, IL-1beta + 
      dexamethasone group, and IL-1beta + EGb group (both high and low dose groups). 
      Reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent 
      assay were used to detect MMP-3 expression. Compared to the MMP-3 mRNA level in 
      the control group, MMP-3 mRNA level significantly increased in the model group (P 
      < 0.05). The application of dexamethasone or EGb significantly decreased the 
      MMP-3 mRNA level (P < 0.05). MMP-3 mRNA and protein levels decreased in the 
      EGb-treated group, especially in the high-dose group, compared to those in the 
      dexamethasone group (P < 0.05). EGb may reduce MMP-3 production during 
      IL-1beta-induced chondrocyte damage and protect chondrocytes to some extent, with 
      better efficacy at high doses.
FAU - Zeng, J Z
AU  - Zeng JZ
AD  - Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, China.
AD  - Department of Orthopaedics, Beijing Luhe Hospital, Capital Medical University, 
      Beijing, China.
FAU - Ma, L F
AU  - Ma LF
AD  - Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, China.
FAU - Meng, H
AU  - Meng H
AD  - Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, China.
FAU - Yu, H M
AU  - Yu HM
AD  - Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, China.
FAU - Zhang, Y K
AU  - Zhang YK
AD  - Department of Orthopaedics, Beijing Luhe Hospital, Capital Medical University, 
      Beijing, China.
FAU - Guo, A
AU  - Guo A
AD  - Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20151228
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chondrocytes/*drug effects/*metabolism/pathology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Gene Expression
MH  - Ginkgo biloba/*chemistry
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Osteoarthritis/genetics/metabolism/pathology
MH  - Plant Extracts/chemistry/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
EDAT- 2016/01/20 06:00
MHDA- 2017/01/12 06:00
CRDT- 2016/01/20 06:00
PHST- 2016/01/20 06:00 [entrez]
PHST- 2016/01/20 06:00 [pubmed]
PHST- 2017/01/12 06:00 [medline]
AID - gmr6895 [pii]
AID - 10.4238/2015.December.23.15 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Dec 28;14(4):18280-6. doi: 10.4238/2015.December.23.15.