PMID- 26784159 OWN - NLM STAT- MEDLINE DCOM- 20161006 LR - 20200225 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 21 IP - 1 DP - 2016 Jan 15 TI - Analysis on the Physicochemical Properties of Ginkgo biloba Leaves after Enzymolysis Based Ultrasound Extraction and Soxhlet Extraction. PG - 97 LID - 10.3390/molecules21010097 [doi] LID - 97 AB - In this study, high performance liquid chromatography (HPLC), ultraviolet (UV), thermagravimetric analyzer (TGA), pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS), and scanning electron microscope (SEM) were used as measurement techniques, contents of chemical composition, pyrolytic products, thermal stability, morphological characterization of Ginkgo biloba leaves (GBL) acted as the index, and physicochemical properties of GBL after enzymolysis based ultrasound extraction (EBUE) and Soxhlet extraction were studied. The detection results of chemical composition revealed that contents of general flavone, soluble protein, soluble total sugar and protein in the GBL declined significantly after EBUE, and contents of polyprenols and crude fat obviously reduced as well after Soxhlet extraction. Py-GC-MS results indicated that total GC contents of micromolecules with carbon less than 12 from 54.0% before EBUE decline to 8.34% after EBUE. Total GC contents of long-chain fatty acids with carbon less than 20 from 43.0% before EBUE reduced to 27.0% after Soxhlet extraction. Thermal stability results showed that GBL after Soxhlet extraction was easier to decompose than GBL before EBUE. SEM results illustrated that surface structure of GBL was damaged severely after EBUE, compared with GBL before EBUE, while organic solvent extraction had little influence on the morphological characterization of GBL after Soxhlet extraction compared with GBL after EBUE. FAU - Zhang, Chang-Wei AU - Zhang CW AD - Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China. 13770752951@163.com. AD - National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China. 13770752951@163.com. AD - Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China. 13770752951@163.com. AD - Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu, China. 13770752951@163.com. FAU - Wang, Cheng-Zhang AU - Wang CZ AD - Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China. wangczlhs@sina.com. AD - National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China. wangczlhs@sina.com. AD - Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China. wangczlhs@sina.com. AD - Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu, China. wangczlhs@sina.com. AD - Institute of New Technology of Forestry, CAF, Beijing 100091, China. wangczlhs@sina.com. FAU - Tao, Ran AU - Tao R AD - Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China. trmoon1949@126.com. AD - National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China. trmoon1949@126.com. AD - Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China. trmoon1949@126.com. AD - Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu, China. trmoon1949@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160115 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Fatty Acids) RN - 0 (Flavones) RN - 0 (Plant Proteins) RN - 0 (Solvents) RN - EC 3.- (Hydrolases) SB - IM MH - Chromatography, High Pressure Liquid MH - Fatty Acids/isolation & purification MH - Flavones/isolation & purification MH - Gas Chromatography-Mass Spectrometry MH - Ginkgo biloba/*chemistry MH - Hydrolases/*chemistry MH - Hydrolysis MH - Liquid-Liquid Extraction/*methods MH - Plant Leaves/*chemistry MH - Plant Proteins/isolation & purification MH - Solvents MH - Sonication PMC - PMC6273774 OTO - NOTNLM OT - Ginkgo biloba leaves OT - Soxhlet extraction OT - enzymolysis OT - physicochemical properties OT - ultrasound COIS- The authors declare no conflict of interest. EDAT- 2016/01/20 06:00 MHDA- 2016/10/08 06:00 PMCR- 2016/01/15 CRDT- 2016/01/20 06:00 PHST- 2015/10/13 00:00 [received] PHST- 2016/01/05 00:00 [revised] PHST- 2016/01/11 00:00 [accepted] PHST- 2016/01/20 06:00 [entrez] PHST- 2016/01/20 06:00 [pubmed] PHST- 2016/10/08 06:00 [medline] PHST- 2016/01/15 00:00 [pmc-release] AID - molecules21010097 [pii] AID - molecules-21-00097 [pii] AID - 10.3390/molecules21010097 [doi] PST - epublish SO - Molecules. 2016 Jan 15;21(1):97. doi: 10.3390/molecules21010097.