PMID- 26785083
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20181202
IS  - 1423-002X (Electronic)
IS  - 0378-7346 (Linking)
VI  - 81
IP  - 4
DP  - 2016
TI  - Associations of Promoter Methylations and mRNA Expressions of MMP-2, MMP-7 and 
      MMP-9 with Primary Fallopian Tube Carcinoma.
PG  - 367-74
LID - 10.1159/000443639 [doi]
AB  - OBJECTIVE: To explore the associations of matrix metalloprotease-2 (MMP-2), MMP-7 
      and MMP-9 methylations and messenger ribonucleic acid (mRNA) expressions with 
      primary fallopian tube carcinoma (PFTC) development and prognosis. METHODS: We 
      recruited 48 patients with PFTC into the case group and 48 healthy individuals 
      into the control group; PFTC tissues and normal fallopian tube tissues were 
      obtained from subjects in both groups. Methylation specific polymerase chain 
      reaction (PCR), reverse transcription PCR and the immunohistochemical method were 
      used to examine methylation, mRNA expressions and protein expressions of MMP-2, 
      MMP-7 and MMP-9, respectively. RESULTS: The methylation rates of MMP-2, MMP-7 and 
      MMP-9 in the case group were significantly lower than those in the control group 
      (all p < 0.05); MMP-2, MMP-7 and MMP-9 protein and mRNA expressions of PFTC 
      tissues were enormously higher than those of normal tissues (all p < 0.05); 
      univariate survival analysis indicated that MMP-2 and MMP-9 methylations and 
      their protein expressions were associated with PFTC prognosis (all p < 0.05), 
      which was further confirmed by the Cox regression model (all p < 0.05). 
      CONCLUSION: The protein and mRNA expressions of MMP-2, MMP-7 and MMP-9 might be 
      related to PFTC, while the methylations and protein expressions of MMP-2 and 
      MMP-9 might be associated with PFTC progression and prognosis.
CI  - (c) 2016 S. Karger AG, Basel.
FAU - Ren, Yuefang
AU  - Ren Y
AD  - Department of Gynecology, Huzhou Maternity and Child Care Hospital, Huzhou, PR 
      China.
FAU - Zhou, Yinjian
AU  - Zhou Y
FAU - Liu, Mingsong
AU  - Liu M
FAU - Zhang, Su
AU  - Zhang S
LA  - eng
PT  - Journal Article
DEP - 20160120
PL  - Switzerland
TA  - Gynecol Obstet Invest
JT  - Gynecologic and obstetric investigation
JID - 7900587
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.23 (Matrix Metalloproteinase 7)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adult
MH  - Aged
MH  - DNA Methylation/*genetics
MH  - Fallopian Tube Neoplasms/enzymology/*genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 2/analysis/*genetics
MH  - Matrix Metalloproteinase 7/analysis/*genetics
MH  - Matrix Metalloproteinase 9/analysis/*genetics
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Prognosis
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA, Messenger/analysis
EDAT- 2016/01/20 06:00
MHDA- 2017/04/18 06:00
CRDT- 2016/01/20 06:00
PHST- 2015/08/19 00:00 [received]
PHST- 2015/12/23 00:00 [accepted]
PHST- 2016/01/20 06:00 [entrez]
PHST- 2016/01/20 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
AID - 000443639 [pii]
AID - 10.1159/000443639 [doi]
PST - ppublish
SO  - Gynecol Obstet Invest. 2016;81(4):367-74. doi: 10.1159/000443639. Epub 2016 Jan 
      20.