PMID- 26786651
OWN - NLM
STAT- MEDLINE
DCOM- 20161025
LR  - 20191210
IS  - 1873-281X (Electronic)
IS  - 1472-9792 (Linking)
VI  - 96
DP  - 2016 Jan
TI  - In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB 
      among HIV-positive subjects.
PG  - 27-30
LID - S1472-9792(15)30037-8 [pii]
LID - 10.1016/j.tube.2015.10.005 [doi]
AB  - BACKGROUND: The recently introduced IFN-gamma release assay (IGRA) has been reported 
      to improve the diagnosis of TB. However, IGRA has suboptimal sensitivity to 
      diagnose TB among HIV co-infected subjects. Apart from IFN-gamma, the pro 
      inflammatory cytokines such as Interleukin-1beta (IL-1beta), Tumor necrosis 
      factor-alpha (TNF-alpha), IL-2, IL-6, IL-8 and IL-12 are also play a major role in 
      mycobacterial infections. This study aimed to analyze these cytokines for 
      detecting active TB among HIV sero positive subjects. MATERIALS AND METHODS: We 
      had prospectively enrolled 53 HIV positive subjects and 55 HIV-TB co-infected 
      patients from India. IGRA was performed by using QuantiFERON TB-Gold In tube 
      (QFT-GIT) method. TB antigen specific IL-1beta, TNF-alpha, IL-2, IL-6, IL-8 and IL-12 
      levels were evaluated by ELISA in plasma harvested from QFT-GIT tubes. RESULTS 
      AND CONCLUSION: The TB antigen specific IL-1beta levels were significantly elevated 
      in HIV-TB co-infected patients compared to HIV positive subjects (p = 0.0004). 
      The specificity of both IL-1beta (50.94%) and QFT-GIT (52.83%) remained similar in 
      HIV positive subjects (p = 0.24). However, IL-1beta had shown higher sensitivity 
      (72.73%) than QFT-GIT (54.55%) to diagnose TB among HIV co-infected patients. 
      Moreover, in culture test positive HIV-TB patients, antigen specific IL-1beta 
      exhibited sensitivity of 84.21%; whereas QFT-GIT exhibited only 57.89% 
      sensitivity. Unlike IFN-gamma (the read out marker of QFT-GIT), antigen specific 
      IL-1beta levels were not influenced by low CD4 counts. The other cytokine levels 
      were not significantly differ between the 2 groups.From this study we concluded 
      that TB antigen specific IL-1beta may be an additional biomarker for active TB 
      diagnosis among HIV positive subjects.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Prabhavathi, Maddineni
AU  - Prabhavathi M
AD  - Department of Immunology, National Institute for Research in Tuberculosis (ICMR), 
      No. 1, Mayor Sathyamoorthy Road, Chetpet, Chennai, 600 031, Tamil Nadu, India. 
      Electronic address: prabha.biochem1@gmail.com.
FAU - Ahamed Kabeer, Basirudeen Syed
AU  - Ahamed Kabeer BS
AD  - Cardiovascular Division, Sidra Medical and Research Center, Doha, Qatar. 
      Electronic address: bkabeer@sidra.org.
FAU - Deenadayalan, Anbarasu
AU  - Deenadayalan A
AD  - Department of Immunology, National Institute for Research in Tuberculosis (ICMR), 
      No. 1, Mayor Sathyamoorthy Road, Chetpet, Chennai, 600 031, Tamil Nadu, India. 
      Electronic address: harianbu1@gmail.com.
FAU - Raja, Alamelu
AU  - Raja A
AD  - Department of Immunology, National Institute for Research in Tuberculosis (ICMR), 
      No. 1, Mayor Sathyamoorthy Road, Chetpet, Chennai, 600 031, Tamil Nadu, India. 
      Electronic address: alameluraja@gmail.com.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20151110
PL  - Scotland
TA  - Tuberculosis (Edinb)
JT  - Tuberculosis (Edinburgh, Scotland)
JID - 100971555
RN  - 0 (Biomarkers)
RN  - 0 (IFNG protein, human)
RN  - 0 (IL1B protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Biomarkers/blood
MH  - CD4 Lymphocyte Count
MH  - *Coinfection
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HIV Infections/blood/*complications/diagnosis/immunology
MH  - Humans
MH  - India
MH  - Interferon-gamma/blood/immunology
MH  - Interferon-gamma Release Tests
MH  - Interleukin-1beta/*blood/immunology
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/*immunology
MH  - Predictive Value of Tests
MH  - Prospective Studies
MH  - Reproducibility of Results
MH  - Tuberculosis/blood/complications/*diagnosis/immunology/microbiology
MH  - Young Adult
EDAT- 2016/01/21 06:00
MHDA- 2016/10/26 06:00
CRDT- 2016/01/21 06:00
PHST- 2015/04/17 00:00 [received]
PHST- 2015/10/04 00:00 [revised]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2016/01/21 06:00 [entrez]
PHST- 2016/01/21 06:00 [pubmed]
PHST- 2016/10/26 06:00 [medline]
AID - S1472-9792(15)30037-8 [pii]
AID - 10.1016/j.tube.2015.10.005 [doi]
PST - ppublish
SO  - Tuberculosis (Edinb). 2016 Jan;96:27-30. doi: 10.1016/j.tube.2015.10.005. Epub 
      2015 Nov 10.