PMID- 26788066
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160120
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2016
DP  - 2016
TI  - Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human 
      Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing.
PG  - 3232859
LID - 10.1155/2016/3232859 [doi]
LID - 3232859
AB  - Vascular ulcers are a serious complication of peripheral vascular disease, 
      especially in diabetics. Several approaches to treat the wounds are proposed but 
      they show poor outcomes and require long healing times. Hepatocyte Growth 
      Factor/Scatter Factor (HGF/SF) is a pleiotropic cytokine exerting many biological 
      activities through the c-Met receptor. This study was aimed at verifying whether 
      HGF/SF influences proliferation, migration, and angiogenesis on mesenchymal stem 
      cells isolated from human arteries (hVW-MSCs). hVW-MSCs were exposed to NIBSC 
      HGF/SF (2.5, 5, 10, and 70 ng/mL) from 6 hrs to 7 days. HGF and c-MET mRNA and 
      protein expression, cell proliferation (Alamar Blue and Ki-67 assay), migration 
      (scratch and transwell assays), and angiogenesis (Matrigel) were investigated. 
      hVW-MSCs displayed stemness features and expressed HGF and c-MET. HGF/SF did not 
      increase hVW-MSC proliferation, whereas it enhanced the cell migration, the 
      formation of capillary-like structures, and the expression of angiogenic markers 
      (vWF, CD31, and KDR). The HGF/SF effects on hVW-MSC migration and angiogenic 
      potential are of great interest to accelerate wound healing process. Local 
      delivery of HGF/SF could therefore improve the healing of unresponsive vascular 
      ulcers.
FAU - Valente, Sabrina
AU  - Valente S
AD  - Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University 
      of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
FAU - Ciavarella, Carmen
AU  - Ciavarella C
AUID- ORCID: 0000-0001-6465-9945
AD  - Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University 
      of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
FAU - Pasanisi, Emanuela
AU  - Pasanisi E
AD  - Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University 
      of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
FAU - Ricci, Francesca
AU  - Ricci F
AD  - Cardiovascular Tissue Bank-Immunohematology and Transfusion Medicine, University 
      Hospital St. Orsola-Malpighi, Polyclinic of Bologna, Via Massarenti 9, 40138 
      Bologna, Italy.
FAU - Stella, Andrea
AU  - Stella A
AD  - Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University 
      of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
FAU - Pasquinelli, Gianandrea
AU  - Pasquinelli G
AUID- ORCID: 0000-0003-1506-1829
AD  - Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University 
      of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20151214
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4691635
EDAT- 2016/01/21 06:00
MHDA- 2016/01/21 06:01
PMCR- 2015/12/14
CRDT- 2016/01/21 06:00
PHST- 2015/04/20 00:00 [received]
PHST- 2015/08/11 00:00 [revised]
PHST- 2015/08/23 00:00 [accepted]
PHST- 2016/01/21 06:00 [entrez]
PHST- 2016/01/21 06:00 [pubmed]
PHST- 2016/01/21 06:01 [medline]
PHST- 2015/12/14 00:00 [pmc-release]
AID - 10.1155/2016/3232859 [doi]
PST - ppublish
SO  - Stem Cells Int. 2016;2016:3232859. doi: 10.1155/2016/3232859. Epub 2015 Dec 14.