PMID- 26788115
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160120
LR  - 20201001
IS  - 1743-7075 (Print)
IS  - 1743-7075 (Electronic)
IS  - 1743-7075 (Linking)
VI  - 13
DP  - 2016
TI  - Effects of selected bioactive food compounds on human white adipocyte function.
PG  - 4
LID - 10.1186/s12986-016-0064-3 [doi]
LID - 4
AB  - BACKGROUND: Previous studies suggest that intake of specific bioactive compounds 
      may have beneficial clinical effects on adipose tissue partly due to their 
      anti-inflammatory and insulin-sensitizing properties. With the overall aim to 
      contribute to better understanding of the mechanisms of selected bioactive 
      nutrients on fat metabolism, we investigated their role on human white adipocyte 
      function. METHODS: The influence of the omega-3-fatty acid docosahexaenoic acid 
      (DHA), the anthocyanin (AC) cyanidin-3-glucoside and its metabolite 
      protocatechuic acid, and the beta-glucan metabolite propionic acid (PI) on 
      adipokine secretion, fatty acid metabolism (lipolysis/lipogenesis) and adipocyte 
      differentiation (lipid accumulation) was studied in human fat cells 
      differentiated in vitro. To investigate possible synergistic, additive or 
      antagonistic effects, DHA was also combined with AC or PI. RESULTS: Each 
      compound, alone or together with DHA, suppressed basal adipocyte lipolysis 
      compared to control treated cells. DHA alone attenuated the secretion of 
      pro-inflammatory adipokines such as chemerin, interleukin-6 (IL-6) and monocyte 
      chemoattractant protein-1 (MCP-1/CCL2), whereas AC suppressed only the latter 
      two. Treatment with PI decreased IL-6, tumour necrosis factor alpha (TNFalpha) and 
      adiponectin secretion. A combination of DHA and AC decreased TNFalpha secretion and 
      increased insulin-stimulated lipogenesis. No effect was found on adipocyte 
      differentiation. At the selected concentrations, none of the compounds was found 
      to be cytotoxic. CONCLUSION: The studied bioactive food compounds or their 
      metabolites have beneficial effects in human primary fat cells measured as 
      decreased basal lipolytic activity and secretion of inflammatory markers. A minor 
      effect was also observed on insulin-stimulated glucose uptake albeit only with 
      the combination of DHA and AC. Taken together, our results may link the reported 
      health benefits of the selected bioactives on metabolic disorders such as insulin 
      resistance, hypertension and dyslipidemia to effects on white adipocytes.
FAU - Bjork, Christel
AU  - Bjork C
AD  - Lipid Laboratory, Department of Medicine, Karolinska Institutet, Huddinge, 
      Stockholm, Sweden ; Department of Medicine, Karolinska Institutet, Lipid 
      Laboratory, Novum, NVS D4, Halsovagen 7, 14186 Stockholm, Sweden.
FAU - Wilhelm, Uta
AU  - Wilhelm U
AD  - Lipid Laboratory, Department of Medicine, Karolinska Institutet, Huddinge, 
      Stockholm, Sweden.
FAU - Mandrup, Susanne
AU  - Mandrup S
AD  - Department of Biochemistry and Molecular Biology, University of Southern Denmark, 
      5230 Odense M, Denmark.
FAU - Larsen, Bjork Ditlev
AU  - Larsen BD
AD  - Department of Biochemistry and Molecular Biology, University of Southern Denmark, 
      5230 Odense M, Denmark.
FAU - Bordoni, Alessandra
AU  - Bordoni A
AD  - Department of Agro-Food Sciences and Technologies, University of Bologna, 
      Bologna, Italy.
FAU - Heden, Per
AU  - Heden P
AD  - Department of Plastic Surgery, Akademikliniken, Stockholm, Sweden.
FAU - Ryden, Mikael
AU  - Ryden M
AD  - Lipid Laboratory, Department of Medicine, Karolinska Institutet, Huddinge, 
      Stockholm, Sweden.
FAU - Arner, Peter
AU  - Arner P
AD  - Lipid Laboratory, Department of Medicine, Karolinska Institutet, Huddinge, 
      Stockholm, Sweden.
FAU - Laurencikiene, Jurga
AU  - Laurencikiene J
AD  - Lipid Laboratory, Department of Medicine, Karolinska Institutet, Huddinge, 
      Stockholm, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20160119
PL  - England
TA  - Nutr Metab (Lond)
JT  - Nutrition & metabolism
JID - 101231644
PMC - PMC4717570
OTO - NOTNLM
OT  - Anthocyanin
OT  - Beta-glucan
OT  - Cyanidin-3-glucoside
OT  - Docosahexaenoic acid
OT  - Inflammation
OT  - Insulin sensitivity
OT  - Lipolysis
OT  - Propionic acid
OT  - Protocatechuic acid
EDAT- 2016/01/21 06:00
MHDA- 2016/01/21 06:01
PMCR- 2016/01/19
CRDT- 2016/01/21 06:00
PHST- 2015/08/13 00:00 [received]
PHST- 2016/01/14 00:00 [accepted]
PHST- 2016/01/21 06:00 [entrez]
PHST- 2016/01/21 06:00 [pubmed]
PHST- 2016/01/21 06:01 [medline]
PHST- 2016/01/19 00:00 [pmc-release]
AID - 64 [pii]
AID - 10.1186/s12986-016-0064-3 [doi]
PST - epublish
SO  - Nutr Metab (Lond). 2016 Jan 19;13:4. doi: 10.1186/s12986-016-0064-3. eCollection 
      2016.