PMID- 26791814
OWN - NLM
STAT- MEDLINE
DCOM- 20171019
LR  - 20181202
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 69
IP  - 2
DP  - 2016 Aug
TI  - Loss of ezrin in human intrahepatic cholangiocarcinoma is associated with ectopic 
      expression of E-cadherin.
PG  - 211-21
LID - 10.1111/his.12931 [doi]
AB  - AIMS: Ezrin connects proteins from the plasma membrane to the subcortical 
      cytoskeleton, and contributes to epithelial integrity by interacting with the 
      cell-cell adhesion molecule E-cadherin. In the liver, ezrin is restricted to 
      cholangiocytes, where it regulates biliary secretory functions. During 
      carcinogenesis, ezrin expression is impaired and associated with enhancement of 
      cell migratory activity in cancer cells; therefore, we aimed to analyse ezrin in 
      cholangiocarcinogenesis. METHODS AND RESULTS: Ezrin expression was evaluated by 
      immunohistochemistry on tissue microarrays from 94 surgical specimens of 
      intrahepatic cholangiocarcinoma (CCA), and correlated with clinicopathological 
      factors and E-cadherin expression. Ezrin function was also analysed in human CCA 
      cell lines. In CCA, ezrin was negative/weakly expressed in 49 cases (52%) and 
      moderately/strongly expressed in 45 cases (48%), mostly in cell cytoplasm. The 
      negative/weak expression of ezrin was more frequent in peripheral than in 
      perihilar CCA (P = 0.002), and was associated with high tumour size (P = 0.001), 
      low mucus secretion (P = 0.042), the presence of satellite nodules (P = 0.024), 
      and ectopic cytoplasmic expression of E-cadherin (P = 0.005). In vitro, silencing 
      of ezrin in CCA cells caused internalization of E-cadherin and favoured cell 
      migration. CONCLUSIONS: Ezrin is down-regulated during cholangiocarcinogenesis, 
      and its loss results in a more aggressive phenotype.
CI  - (c) 2016 John Wiley & Sons Ltd.
FAU - Guedj, Nathalie
AU  - Guedj N
AD  - Service d'anatomie pathologique Hopital Beaujon, Clichy, France.
AD  - INSERM, UMR 1149, Centre de Recherche sur l'Inflammation, Paris, France.
FAU - Vaquero, Javier
AU  - Vaquero J
AD  - INSERM, UMR_S 938, Paris, France.
AD  - Sorbonne Universites, UPMC Universite Paris 06, UMR_S 938, Centre de Recherche 
      Saint-Antoine, Paris, France.
FAU - Claperon, Audrey
AU  - Claperon A
AD  - INSERM, UMR_S 938, Paris, France.
AD  - Sorbonne Universites, UPMC Universite Paris 06, UMR_S 938, Centre de Recherche 
      Saint-Antoine, Paris, France.
FAU - Mergey, Martine
AU  - Mergey M
AD  - INSERM, UMR_S 938, Paris, France.
AD  - Sorbonne Universites, UPMC Universite Paris 06, UMR_S 938, Centre de Recherche 
      Saint-Antoine, Paris, France.
FAU - Chretien, Yves
AU  - Chretien Y
AD  - INSERM, UMR_S 938, Paris, France.
AD  - Sorbonne Universites, UPMC Universite Paris 06, UMR_S 938, Centre de Recherche 
      Saint-Antoine, Paris, France.
FAU - Paradis, Valerie
AU  - Paradis V
AD  - Service d'anatomie pathologique Hopital Beaujon, Clichy, France.
AD  - INSERM, UMR 1149, Centre de Recherche sur l'Inflammation, Paris, France.
FAU - Fouassier, Laura
AU  - Fouassier L
AD  - INSERM, UMR_S 938, Paris, France.
AD  - Sorbonne Universites, UPMC Universite Paris 06, UMR_S 938, Centre de Recherche 
      Saint-Antoine, Paris, France.
LA  - eng
PT  - Journal Article
DEP - 20160301
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (ezrin)
SB  - IM
MH  - Aged
MH  - Antigens, CD
MH  - Bile Duct Neoplasms/diagnosis/*metabolism/pathology/surgery
MH  - Biomarkers, Tumor/*metabolism
MH  - Cadherins/*metabolism
MH  - Carcinogenesis
MH  - Cell Line, Tumor
MH  - Cell Membrane/metabolism
MH  - Cell Movement
MH  - Cholangiocarcinoma/diagnosis/*metabolism/pathology/surgery
MH  - Cytoskeletal Proteins/*metabolism
MH  - Down-Regulation
MH  - Ectopic Gene Expression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Liver/metabolism/pathology
MH  - Liver Neoplasms/diagnosis/*metabolism/pathology/surgery
MH  - Tissue Array Analysis
OTO - NOTNLM
OT  - E-cadherin
OT  - cellular biology
OT  - cholangiocarcinoma
OT  - ezrin
OT  - immunohistochemistry
EDAT- 2016/01/23 06:00
MHDA- 2017/10/20 06:00
CRDT- 2016/01/22 06:00
PHST- 2015/04/17 00:00 [received]
PHST- 2016/01/14 00:00 [accepted]
PHST- 2016/01/22 06:00 [entrez]
PHST- 2016/01/23 06:00 [pubmed]
PHST- 2017/10/20 06:00 [medline]
AID - 10.1111/his.12931 [doi]
PST - ppublish
SO  - Histopathology. 2016 Aug;69(2):211-21. doi: 10.1111/his.12931. Epub 2016 Mar 1.