PMID- 26792938
OWN - NLM
STAT- MEDLINE
DCOM- 20161104
LR  - 20220311
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 94
IP  - 2
DP  - 2016 Feb
TI  - Proinflammatory Response of Human Trophoblastic Cells to Brucella abortus 
      Infection and upon Interactions with Infected Phagocytes.
PG  - 48
LID - 10.1095/biolreprod.115.131706 [doi]
AB  - Trophoblasts are targets of infection by Brucella spp. but their role in the 
      pathophysiology of pregnancy complications of brucellosis is unknown. Here we 
      show that Brucella abortus invades and replicates in the human trophoblastic cell 
      line Swan-71 and that the intracellular survival of the bacterium depends on a 
      functional virB operon. The infection elicited significant increments of 
      interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), and IL6 secretion, 
      but levels of IL1beta and tumor necrosis factor-alpha (TNF-alpha) did not vary 
      significantly. Such proinflammatory response was not modified by the absence of 
      the Brucella TIR domain-containing proteins BtpA and BtpB. The stimulation of 
      Swan-71 cells with conditioned medium (CM) from B. abortus-infected human 
      monocytes (THP-1 cells) or macrophages induced a significant increase of IL8, 
      MCP-1 and IL6 as compared to stimulation with CM from non-infected cells. Similar 
      results were obtained when stimulation was performed with CM from infected 
      neutrophils. Neutralization studies showed that IL1beta and/or TNF-alpha mediated 
      the stimulating effects of CM from infected phagocytes. Reciprocally, stimulation 
      of monocytes and neutrophils with CM from Brucella-infected trophoblasts 
      increased IL8 and/or IL6 secretion. These results suggest that human trophoblasts 
      may provide a local inflammatory environment during B. abortus infections either 
      through a direct response to the pathogen or through interactions with 
      monocytes/macrophages or neutrophils, potentially contributing to the pregnancy 
      complications of brucellosis.
CI  - (c) 2016 by the Society for the Study of Reproduction, Inc.
FAU - Fernandez, Andrea G
AU  - Fernandez AG
AD  - Instituto de Estudios de la Inmunidad Humoral (CONICET/UBA), Facultad de Farmacia 
      y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Ferrero, Mariana C
AU  - Ferrero MC
AD  - Instituto de Estudios de la Inmunidad Humoral (CONICET/UBA), Facultad de Farmacia 
      y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Hielpos, M Soledad
AU  - Hielpos MS
AD  - Instituto de Estudios de la Inmunidad Humoral (CONICET/UBA), Facultad de Farmacia 
      y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Fossati, Carlos A
AU  - Fossati CA
AD  - Instituto de Estudios Inmunologicos y Fisiopatologicos (UNLP-CONICET), Facultad 
      de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
FAU - Baldi, Pablo C
AU  - Baldi PC
AD  - Instituto de Estudios de la Inmunidad Humoral (CONICET/UBA), Facultad de Farmacia 
      y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina 
      pablobal@ffyb.uba.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160120
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - *Brucella abortus
MH  - Brucellosis/metabolism/*pathology
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/metabolism/*microbiology/pathology
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Macrophages/metabolism/microbiology/pathology
MH  - Monocytes/metabolism/microbiology/pathology
MH  - Phagocytes/metabolism/*microbiology/pathology
MH  - Trophoblasts/metabolism/*microbiology/pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Brucella
OT  - cellular interactions
OT  - inflammatory response
OT  - trophoblast
EDAT- 2016/01/23 06:00
MHDA- 2016/11/05 06:00
CRDT- 2016/01/22 06:00
PHST- 2015/07/02 00:00 [received]
PHST- 2016/01/07 00:00 [accepted]
PHST- 2016/01/22 06:00 [entrez]
PHST- 2016/01/23 06:00 [pubmed]
PHST- 2016/11/05 06:00 [medline]
AID - biolreprod.115.131706 [pii]
AID - 10.1095/biolreprod.115.131706 [doi]
PST - ppublish
SO  - Biol Reprod. 2016 Feb;94(2):48. doi: 10.1095/biolreprod.115.131706. Epub 2016 Jan 
      20.