PMID- 26795299
OWN - NLM
STAT- MEDLINE
DCOM- 20160823
LR  - 20191209
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 97
IP  - 4
DP  - 2016 Apr
TI  - Modification of the internal ribosome entry site element impairs the growth of 
      foot-and-mouth disease virus in porcine-derived cells.
PG  - 901-911
LID - 10.1099/jgv.0.000406 [doi]
AB  - The 5' untranslated region (5'UTR) of foot-and-mouth disease virus (FMDV) 
      contains an internal ribosome entry site (IRES) that facilitates translation 
      initiation of the viral ORF in a 5' (m7GpppN) cap-independent manner. IRES 
      elements are responsible for the virulence phenotypes of several enteroviruses. 
      Here, we constructed a chimeric virus in which the IRES of FMDV was completely 
      replaced with that of bovine rhinitis B virus (BRBV) in an infectious clone of 
      serotype O FMDV. The resulting IRES-replaced virus, FMDV(BRBV), replicated as 
      efficiently as WT FMDV in hamster-derived BHK-21 cells, but was restricted for 
      growth in porcine-derived IBRS-2, PK-15 and SK-6 cells, which are susceptible to 
      WT FMDV. To identify the genetic determinants of FMDV underlying this altered 
      cell tropism, a series of IRES-chimeric viruses were constructed in which each 
      domain of the FMDV IRES was replaced with its counterpart from the BRBV IRES. The 
      replication kinetics of these chimeric viruses in different cell lines revealed 
      that the growth restriction phenotype in porcine-derived cells was produced after 
      the replacement of domain 3 or 4 in the FMDV IRES. Furthermore, the change in 
      FMDV cell tropism due to IRES replacement in porcine-derived cells was mainly 
      attributed to a decline in cell-specific IRES translation initiation efficiency. 
      These findings demonstrate that IRES domains 3 and 4 of FMDV are novel 
      cell-specific cis-elements for viral replication in vitro and suggest that 
      IRES-mediated translation determines the species specificity of FMDV infection in 
      vivo.
FAU - Sun, Chao
AU  - Sun C
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
FAU - Yang, Decheng
AU  - Yang D
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
FAU - Gao, Rongyuan
AU  - Gao R
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
FAU - Liang, Te
AU  - Liang T
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
FAU - Wang, Haiwei
AU  - Wang H
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
FAU - Zhou, Guohui
AU  - Zhou G
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
FAU - Yu, Li
AU  - Yu L
AD  - Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, PRChina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160120
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Internal Ribosome Entry Sites)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chimera/genetics
MH  - Cricetulus
MH  - Epithelial Cells/pathology/virology
MH  - Foot-and-Mouth Disease Virus/*genetics/growth & development
MH  - *Gene Expression Regulation, Viral
MH  - Herpesvirus 1, Bovine/*genetics
MH  - Host Specificity
MH  - *Internal Ribosome Entry Sites
MH  - Kidney/pathology/virology
MH  - Molecular Sequence Data
MH  - Peptide Chain Initiation, Translational
MH  - RNA, Viral/genetics/metabolism
MH  - Reassortant Viruses/*genetics/growth & development
MH  - Replicon
MH  - Ribosomes
MH  - Swine
MH  - *Viral Tropism
MH  - Virus Replication
PMC - PMC4854365
EDAT- 2016/01/23 06:00
MHDA- 2016/08/24 06:00
PMCR- 2017/04/01
CRDT- 2016/01/23 06:00
PHST- 2016/01/23 06:00 [entrez]
PHST- 2016/01/23 06:00 [pubmed]
PHST- 2016/08/24 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 000406 [pii]
AID - 10.1099/jgv.0.000406 [doi]
PST - ppublish
SO  - J Gen Virol. 2016 Apr;97(4):901-911. doi: 10.1099/jgv.0.000406. Epub 2016 Jan 20.