PMID- 26795730 OWN - NLM STAT- MEDLINE DCOM- 20161014 LR - 20181113 IS - 1756-9966 (Electronic) IS - 0392-9078 (Print) IS - 0392-9078 (Linking) VI - 35 DP - 2016 Jan 22 TI - Eliciting cytotoxic T lymphocytes against human laryngeal cancer-derived antigens: evaluation of dendritic cells pulsed with a heat-treated tumor lysate and other antigen-loading strategies for dendritic-cell-based vaccination. PG - 18 LID - 10.1186/s13046-016-0295-1 [doi] LID - 18 AB - BACKGROUND: Dendritic cells (DCs) have been used successfully in clinical pilot studies. However, tumor-specific immunity and clinical responses were only induced in certain cancer patients. It has been well documented that immunotherapy efficacy can be optimized for responses using antigen pulsing. METHODS: The human laryngeal squamous cell cancer (LSCC) cell line SNU899 was used to evaluate the in vitro anti-tumor efficacy of three different preparations of dendritic cell (DC) vaccines consisting of either whole tumor cells or their derivatives including: i) DCs pulsed with a tumor cell supernatant (DC-TCS), ii) DCs pulsed with whole-cell tumor stressed lysate (DC-TSL), and iii) DCs pulsed with irradiated tumor cells (DC-ITC). RESULTS: Our results showed that DC-TSL is an effective source of tumor-associated antigens (TAAs) for pulsing DCs. DC-TSL induced the highest expansion of TAA-specific T cells, the strongest Th1 cytokine response, and the most potent cytotoxic T lymphocyte (CTL) activity. DC-TCS and DC-ITC inhibited T cell activation but induced a certain extent of CTL activity. CONCLUSIONS: These data suggest that DC-TSL is a more potent inducer of antitumor immunity against laryngeal cancer than other antigen-loading strategies using whole tumor cell materials. This strategy provides an alternative approach for DC-based immunotherapy for laryngeal cancer. FAU - Wei, Fan-Qin AU - Wei FQ AD - Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. 327826543@qq.com. AD - Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. 327826543@qq.com. AD - Department of Otorhinolaryngology Head and Neck Surgery, the Sixth Affiliated Hospital of Sun Yat-Sen University, Yuancun Second Cross Road 26#, Guangzhou, 510655, Guangdong, P.R. China. 327826543@qq.com. FAU - Sun, Wei AU - Sun W AD - Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. sunw2@mail2.sysu.edu.cn. FAU - Wong, Thian-Sze AU - Wong TS AD - Department of Surgery, The University of Hong Kong, Pokfulam Road 102#, Hong Kong, P.R. China. thiansze@gmail.com. FAU - Gao, Wei AU - Gao W AD - Department of Surgery, The University of Hong Kong, Pokfulam Road 102#, Hong Kong, P.R. China. gaoweiwayne@gmail.com. FAU - Wen, Yi-Hui AU - Wen YH AD - Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. yihui-wen@hotmail.com. AD - Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. yihui-wen@hotmail.com. FAU - Wei, Jia-Wei AU - Wei JW AD - Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weijiaw@mail2.sysu.edu.cn. AD - Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weijiaw@mail2.sysu.edu.cn. FAU - Wei, Yi AU - Wei Y AD - Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weiyi-009@163.com. AD - Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. weiyi-009@163.com. FAU - Wen, Wei-Ping AU - Wen WP AD - Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. wenwp@mail.sysu.edu.cn. AD - Institute of Otorhinolaryngology Head and Neck Surgery, Sun Yat-sen University, 2nd Zhongshan Road 58#, Guangzhou, 510080, Guangdong, P.R. China. wenwp@mail.sysu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160122 PL - England TA - J Exp Clin Cancer Res JT - Journal of experimental & clinical cancer research : CR JID - 8308647 RN - 0 (Antigens, Neoplasm) RN - 0 (Cancer Vaccines) RN - 0 (Cell Extracts) SB - IM MH - Antigen Presentation MH - Antigens, Neoplasm/*immunology MH - Cancer Vaccines/immunology MH - Carcinoma, Squamous Cell/*immunology/therapy MH - Cell Culture Techniques MH - Cell Extracts/immunology MH - Cell Line, Tumor MH - Dendritic Cells/cytology/*immunology MH - Humans MH - Immunotherapy/methods MH - Laryngeal Neoplasms/*immunology/therapy MH - T-Lymphocytes, Cytotoxic/*immunology PMC - PMC4722756 EDAT- 2016/01/23 06:00 MHDA- 2016/10/16 06:00 PMCR- 2016/01/22 CRDT- 2016/01/23 06:00 PHST- 2015/11/11 00:00 [received] PHST- 2016/01/17 00:00 [accepted] PHST- 2016/01/23 06:00 [entrez] PHST- 2016/01/23 06:00 [pubmed] PHST- 2016/10/16 06:00 [medline] PHST- 2016/01/22 00:00 [pmc-release] AID - 10.1186/s13046-016-0295-1 [pii] AID - 295 [pii] AID - 10.1186/s13046-016-0295-1 [doi] PST - epublish SO - J Exp Clin Cancer Res. 2016 Jan 22;35:18. doi: 10.1186/s13046-016-0295-1.